Development of a highly sensitive fluorescence probe for hydrogen peroxide.

Abstract

Hydrogen peroxide is believed to play a role in cellular signal transduction by reversible oxidation of proteins. Here, we report the design and synthesis of a novel fluorescence probe for hydrogen peroxide, utilizing a photoinduced electron transfer strategy based on benzil chemistry to control the fluorescence. The practical value of this highly sensitive and selective fluorescence probe, NBzF, was confirmed by its application to imaging of hydrogen peroxide generation in live RAW 264.7 macrophages. NBzF was also employed for live cell imaging of hydrogen peroxide generated as a signaling molecule in A431 human epidermoid carcinoma cells.

• Authors: Masahiro Abo, Yasuteru Urano, Kenjiro Hanaoka, Takuya Terai, Toru Komatsu, Tetsuo Nagano
• Journal: Journal of the American Chemical Society (J Am Chem Soc) Vol. 133 Issue 27 Pg. 10629-37 (Jul 13 2011) ISSN: 1520-5126 [Electronic] United States
• PMID: 21692459 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• 5-benzoylcarbonylfluorescein
• Fluoresceins
• Fluorescent Dyes
• Hydrogen Peroxide

Topics

• Animals
• Cell Line, Tumor
• Electron Transport
• Fluoresceins (analysis, chemistry)
• Fluorescence
• Fluorescent Dyes (chemistry)
• Humans
• Hydrogen Peroxide (analysis)
• Macrophages (chemistry)
• Mice