Hypoxia-induced cisplatin resistance is reversible and growth rate independent in lung cancer cells.

Abstract

Hypoxia frequently occurs in solid tumors and is known to contribute to chemotherapy resistance. However, the mechanisms leading to chemotherapy resistance are not entirely known. We investigated hypoxia-induced resistance to cisplatin in NSCLC cell lines. We show that chronic moderate hypoxia induced resistance to cisplatin in NSCLC cells without involvement of selection pressure. Our data suggest that stabilization of the hypoxia-inducible factor 1 alpha and down-regulation of the pro-apoptotic protein BAX play a role in this process. Furthermore, we provide evidence that hypoxia-induced resistance to cisplatin is not due to the reduced growth rate of cancer cells under hypoxic conditions.

• Authors: Christoph Wohlkoenig, Katharina Leithner, Alexander Deutsch, Andelko Hrzenjak, Andrea Olschewski, Horst Olschewski
• Journal: Cancer letters (Cancer Lett) Vol. 308 Issue 2 Pg. 134-43 (Sep 28 2011) ISSN: 1872-7980 [Electronic] Ireland
• PMID: 21669489 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Antineoplastic Agents
• Cisplatin

Topics

• Antineoplastic Agents (pharmacology)
• Apoptosis
• Blotting, Western
• Cell Hypoxia
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Cell Survival
• Cisplatin (pharmacology)
• Drug Resistance, Neoplasm
• Humans
• Lung Neoplasms (pathology)
• Reverse Transcriptase Polymerase Chain Reaction