Evaluation of hepatocyte growth factor plasmid therapeutic effect by 99mTc-hexakis-2-methyoxy-isobutylisonitrile blood flow scintigraphy in a rat model of hind limb ischemia.

Abstract

OBJECTIVE:

This study was conducted in an attempt to use blood flow scintigraphy with 99mTc-hexakis-2-methyoxy-isobutylisonitrile (99mTc-MIBI) for the evaluation of the angiogenic effect of hepatocyte growth factor (HGF) plasmid in a rat model of hind limb ischemia.

MATERIALS AND METHODS:

The femoral artery of the left hind limb of each rat was ligated to create a model of hind limb ischemia. Three weeks later, HGF plasmid (1.5 mg/1.1 ml/body) or saline (1.1 ml/body) was administered intramuscularly into three sites of the ischemic hind limb. Two and 4 weeks after the treatment, blood flow through the hind limb was measured by 99mTc-MIBI scintigraphy. In addition, the number of capillary endothelial cells obtained by immunostaining for CD31 was counted.

RESULTS:

After 99mTc-MIBI scintigraphy in the HGF plasmid-treated group, the blood flow ratio increased significantly from the pretreatment ratio 63.8 to 73.4%, 2 weeks after treatment (P<0.05) and to 97.8%, 4 weeks after treatment (P<0.05). The number of CD31-positive endothelial cells was significantly higher in the HGF plasmid-treated group than in the control group.

CONCLUSIONS:

The experimental study using a rat model of hind limb ischemia showed usefulness of 99mTc-MIBI scintigraphy to evaluate the angiogenic effect of HGF plasmid treatment.

• Authors: Akio Nagano, Takao Komatsuno, Hiroyuki Kasahara, Takao Minamizawa, Takuji Yamagami, Chio Okuyama, Tsunehiko Nishimura
• Journal: Nuclear medicine communications (Nucl Med Commun) Vol. 32 Issue 9 Pg. 818-23 (Sep 2011) ISSN: 1473-5628 [Electronic] England
• PMID: 21633313 (Publication Type: Journal Article)

Chemical References

• Angiogenesis Modulating Agents
• Platelet Endothelial Cell Adhesion Molecule-1
• Hepatocyte Growth Factor
• Technetium Tc 99m Sestamibi

Topics

• Angiogenesis Modulating Agents (pharmacology, therapeutic use)
• Animals
• Blood Circulation (drug effects)
• Disease Models, Animal
• Endothelial Cells (diagnostic imaging, drug effects, metabolism, pathology)
• Extremities (blood supply, diagnostic imaging)
• Hepatocyte Growth Factor (genetics)
• Humans
• Ischemia (diagnostic imaging, drug therapy, pathology, physiopathology)
• Plasmids (pharmacology, therapeutic use)
• Platelet Endothelial Cell Adhesion Molecule-1 (metabolism)
• Radionuclide Imaging
• Rats
• Rats, Sprague-Dawley
• Technetium Tc 99m Sestamibi
• Treatment Outcome