HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Dose-related induction of hepatic preneoplastic lesions by diethylnitrosamine in C57BL/6 mice.

Abstract
The C57BL/6 mouse strain (or derivation of this strain) is used as a background for many transgenic mouse models. This strain has a relatively low susceptibility to chemically induced hepatocarcinogenesis compared with other commonly used experimental mouse strains. In the present study, the authors treated C57BL/6 mice with 25, 50, and 75 mg/kg of diethylnitrosamine (DEN) for 4 or 8 weeks by intraperitoneal injection to investigate the dose-response pattern of preneoplastic and neoplastic lesion formation in the liver. DEN induced preneoplastic lesions and cytokeratin 8/18-positive foci in a dose-dependent manner. In the 75 mg/kg for 8 weeks treatment group, hepatocellular adenoma, cholangioma and hemangioma, and cytokeratin 19-positive foci were also induced, but a significant decrease in body weight was observed. The suitable DEN treatment range for this strain was concluded to be from 75 mg/kg for 4 weeks (total amount = 300 mg/kg) to 50 mg/kg for 8 weeks (total amount = 400 mg/kg). These results should prove useful for future studies investigating hepatocarcinogenesis in both the background C57BL/6 strain and other transgenic mouse models derived from it.
AuthorsMasahiko Kushida, Lisa M Kamendulis, Tyler J Peat, James E Klaunig
JournalToxicologic pathology (Toxicol Pathol) Vol. 39 Issue 5 Pg. 776-86 (Aug 2011) ISSN: 1533-1601 [Electronic] United States
PMID21628716 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
Chemical References
  • Keratin-19
  • Diethylnitrosamine
Topics
  • Animals
  • Carcinogenicity Tests (methods)
  • Diethylnitrosamine (administration & dosage, toxicity)
  • Disease Models, Animal
  • Dose-Response Relationship, Drug
  • Immunohistochemistry
  • Keratin-19 (metabolism)
  • Liver (anatomy & histology, drug effects, metabolism, pathology)
  • Liver Neoplasms, Experimental (chemically induced, metabolism, pathology)
  • Mice
  • Mice, Inbred C57BL
  • Organ Size (drug effects)
  • Precancerous Conditions (chemically induced, metabolism, pathology)
  • Random Allocation

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: