Abstract |
The C57BL/6 mouse strain (or derivation of this strain) is used as a background for many transgenic mouse models. This strain has a relatively low susceptibility to chemically induced hepatocarcinogenesis compared with other commonly used experimental mouse strains. In the present study, the authors treated C57BL/6 mice with 25, 50, and 75 mg/kg of diethylnitrosamine (DEN) for 4 or 8 weeks by intraperitoneal injection to investigate the dose-response pattern of preneoplastic and neoplastic lesion formation in the liver. DEN induced preneoplastic lesions and cytokeratin 8/18-positive foci in a dose-dependent manner. In the 75 mg/kg for 8 weeks treatment group, hepatocellular adenoma, cholangioma and hemangioma, and cytokeratin 19-positive foci were also induced, but a significant decrease in body weight was observed. The suitable DEN treatment range for this strain was concluded to be from 75 mg/kg for 4 weeks (total amount = 300 mg/kg) to 50 mg/kg for 8 weeks (total amount = 400 mg/kg). These results should prove useful for future studies investigating hepatocarcinogenesis in both the background C57BL/6 strain and other transgenic mouse models derived from it.
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Authors | Masahiko Kushida, Lisa M Kamendulis, Tyler J Peat, James E Klaunig |
Journal | Toxicologic pathology
(Toxicol Pathol)
Vol. 39
Issue 5
Pg. 776-86
(Aug 2011)
ISSN: 1533-1601 [Electronic] United States |
PMID | 21628716
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Chemical References |
- Keratin-19
- Diethylnitrosamine
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Topics |
- Animals
- Carcinogenicity Tests
(methods)
- Diethylnitrosamine
(administration & dosage, toxicity)
- Disease Models, Animal
- Dose-Response Relationship, Drug
- Immunohistochemistry
- Keratin-19
(metabolism)
- Liver
(anatomy & histology, drug effects, metabolism, pathology)
- Liver Neoplasms, Experimental
(chemically induced, metabolism, pathology)
- Mice
- Mice, Inbred C57BL
- Organ Size
(drug effects)
- Precancerous Conditions
(chemically induced, metabolism, pathology)
- Random Allocation
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