Abstract |
Intracellular vesicle trafficking is the principal transportation system in eukaryotic cells, and is considered to be involved in a variety of processes related to cell proliferation. A protein named alpha-taxilin has been identified as a binding partner of the syntaxin family, which coordinates intracellular vesicle trafficking. To clarify the role of alpha-taxilin in renal cell carcinoma (RCC), we investigated alpha-taxilin protein expression in clear cell RCC tissues. We analyzed alphataxilin protein in matched sets of tumor and non- tumor tissues from the surgical specimens of 52 Japanese RCC patients by Western blotting. We also studied the relation between alpha-taxilin protein expression in tumor tissues and various clinicopathological features. The alpha-taxilin protein level was higher in tumor tissues than in non- tumor tissues (P < 0.05). Increased expression of alpha-taxilin protein in primary tumors was related to local invasion (P < 0.001), pathological vessel invasion (P < 0.001), and metastasis (P < 0.0001). Kaplan-Meier plots of survival for patients with low versus high alpha-taxilin expression revealed that high expression in tumor tissues was associated with shorter overall survival in all patients (P < 0.05) and with shorter disease-free survival in patients without metastasis (P < 0.01). These findings suggest that alpha-taxilin influences the metastatic and invasive potential of RCC.
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Authors | Tomoko Mashidori, Hiromichi Shirataki, Takao Kamai, Fumihiko Nakamura, Ken-Ichiro Yoshida |
Journal | Biomedical research (Tokyo, Japan)
(Biomed Res)
Vol. 32
Issue 2
Pg. 103-10
(Apr 2011)
ISSN: 1880-313X [Electronic] Japan |
PMID | 21551945
(Publication Type: Journal Article)
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Chemical References |
- Biomarkers, Tumor
- Qa-SNARE Proteins
- TXLNA protein, human
- Vesicular Transport Proteins
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Topics |
- Aged
- Asian People
- Biomarkers, Tumor
(genetics, metabolism)
- Blotting, Western
- Carcinoma, Renal Cell
- Cell Proliferation
- Disease-Free Survival
- Female
- Gene Expression
- Humans
- Kaplan-Meier Estimate
- Kidney Neoplasms
(genetics, metabolism, mortality, pathology)
- Male
- Middle Aged
- Neoplasm Invasiveness
- Prognosis
- Qa-SNARE Proteins
(genetics, metabolism)
- Vesicular Transport Proteins
(genetics, metabolism)
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