Analysis of RNA from brush cytology detects changes in B2M, CYP1B1 and KRT17 levels with OSCC in tobacco users.

Abstract

RNA expression analysis of oral keratinocytes can be used to detect early oral cancer, but a limitation is the inability to obtain high quality RNA from oral tissue without using biopsies. While oral cytology cell samples can be obtained from patients in a minimally invasive manner, they have not been validated for quantitative analysis of RNA expression. Earlier we showed RNA from brush cytology of hamster Oral Squamous Cell Carcinoma (OSCC) demonstrated differential expression of B2M and CYP1B1 using real time RT-PCR in a dibenz[a,I]pyrene, tobacco carcinogen, induced model of this disease. Here we show reproducibility of this approach to measuring gene expression in humans. Cytology brush samples from 12 tobacco and betel related OSCC and 17 nonmalignant oral lesions revealed B2M mRNA was enriched in tumor samples while CYP1B1 mRNA was reduced, similar to what was seen in the model system. Additionally, we showed that KRT17 mRNA, a gene linked to OSCC in another brush cytology study, was also enriched in OSCC versus nonmalignant lesions, again supporting the promise of using RNA from brush oral cytology to reproducibly monitor oral gene expression.

• Authors: Antonia Kolokythas, Joel L Schwartz, Kristen B Pytynia, Suchismita Panda, Mike Yao, Brian Homann, Herve Y Sroussi, Joel B Epstein, Sara C Gordon, Guy R Adami
• Journal: Oral oncology (Oral Oncol) Vol. 47 Issue 6 Pg. 532-6 (Jun 2011) ISSN: 1879-0593 [Electronic] England
• PMID: 21549635 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier Ltd. All rights reserved.

Chemical References

• Keratin-17
• RNA, Messenger
• RNA, Neoplasm
• beta 2-Microglobulin
• Aryl Hydrocarbon Hydroxylases
• CYP1B1 protein, human
• Cytochrome P-450 CYP1B1

Topics

• Adult
• Aged
• Animals
• Aryl Hydrocarbon Hydroxylases (genetics, metabolism)
• Carcinoma, Squamous Cell (metabolism, pathology)
• Cricetinae
• Cytochrome P-450 CYP1B1
• Female
• Humans
• Keratin-17 (genetics, metabolism)
• Male
• Middle Aged
• Mouth Neoplasms (metabolism, pathology)
• RNA, Messenger (metabolism)
• RNA, Neoplasm (metabolism)
• Reproducibility of Results
• Smoking (adverse effects)
• Young Adult
• beta 2-Microglobulin (genetics, metabolism)