Use of a single-tube nested real-time PCR assay to facilitate the early diagnosis of acute Q fever.

Abstract

We have developed a single-tube nested real-time PCR (STN-RT PCR) assay using the repetitive, transposon-like element IS1111 as the DNA target to facilitate early diagnosis of acute Q fever. The use of our proposed diagnostic procedures, including IgM detection by serology and the STN-RT PCR assay, significantly increased the diagnostic sensitivity for Q fever to 78%, compared to 29% when serology alone was used for subjects providing mainly acute-phase blood samples.

• Authors: Min-Yi Hou, Min-Nan Hung, Po-Shan Lin, Yung-Chung Wang, Chien-Chou Lin, Pei-Yun Shu, Wen-Yi Shih, Ho-Sheng Wu, Li-Jen Lin
• Journal: Japanese journal of infectious diseases (Jpn J Infect Dis) Vol. 64 Issue 2 Pg. 161-2 ( 2011) ISSN: 1884-2836 [Electronic] Japan
• PMID: 21519134 (Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA Transposable Elements
• DNA, Bacterial

Topics

• Bacteriological Techniques (methods)
• DNA Transposable Elements
• DNA, Bacterial (genetics)
• Early Diagnosis
• Humans
• Molecular Diagnostic Techniques (methods)
• Polymerase Chain Reaction (methods)
• Q Fever (diagnosis)
• Sensitivity and Specificity
• Serologic Tests (methods)