Heterogeneity of macrophage populations and expression of galectin-3 in cutaneous wound healing in rats.

Abstract

The aim of this study was to investigate the properties of macrophages that infiltrated the sites of cutaneous wound healing in rats between 1 and 26 days post wounding (dpw). During the inflammation phase (1-3 dpw), ED1(+) (CD68(+)) macrophages with enhanced lysosomal activity dominated. From 5 to 7 dpw there was formation of granulation tissue as indicated by the presence of myofibroblasts expressing α-smooth muscle actin. At this stage, ED2(+) (CD163(+)) macrophages, capable of producing inflammatory factors, were dominant. The majority of ED1(+) macrophages expressed galectin-3, a regulator of fibrosis. Corresponding to the increased numbers of ED1(+) and ED2(+) macrophages at 3-9 dpw, there was increased expression of genes encoding transforming growth factor-β1 (a major fibrogenic factor), monocyte chemoattractant protein-1 and colony stimulating factor-1. These macrophage-related factors might contribute to inflammation and formation of granulation tissue. OX6(+) macrophages expressing class II molecules of the major histocompatibility complex became predominant in the healing stages (15-26 dpw), indicating important roles for antigen-presenting cells in tissue remodelling. The OX6(+) macrophages were most likely derived from ED1(+) macrophages. The results of this study show that infiltration of phenotypically- and functionally-distinct macrophage populations characterizes different stages of the wound healing process.

• Authors: V Juniantito, T Izawa, E Yamamoto, F Murai, M Kuwamura, J Yamate
• Journal: Journal of comparative pathology (J Comp Pathol) Vol. 145 Issue 4 Pg. 378-89 (Nov 2011) ISSN: 1532-3129 [Electronic] England
• PMID: 21435650 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier Ltd. All rights reserved.

Chemical References

• Antigens, CD
• Antigens, Differentiation, Myelomonocytic
• CD163 antigen
• CD68 protein, rat
• Galectin 3
• Histocompatibility Antigens Class II
• Receptors, Cell Surface

Topics

• Animals
• Antigens, CD (analysis)
• Antigens, Differentiation, Myelomonocytic (analysis)
• Cell Count
• Cell Lineage
• Fibrosis
• Galectin 3 (biosynthesis, genetics)
• Granulation Tissue (metabolism)
• Histocompatibility Antigens Class II (analysis)
• Macrophages (classification, metabolism)
• Male
• Rats
• Rats, Sprague-Dawley
• Receptors, Cell Surface (analysis)
• Skin (injuries, metabolism, pathology)
• Wound Healing (physiology)