[Simultaneously determination of atropine sulphate, diphenhydramine hydrochloride, capsaicin and dihydrocapsaicin in pain-relieving plaster for arthritis by RP-HPLC].

Abstract

OBJECTIVE:

To establish RP-HPLC method for determination of atropine sulphate, diphenhydramine hydrochloride, capsaicin and dihydrocapsaicin in pain-relieving plaster for arthritis.

METHOD:

The sample were separated on an Alltima C18 Column (4.6 mm x 250 mm, 5 microm) with the moblie phase of CH3 CN-0.1% H3 PO4. Flow rate was 1 mL x min(-1). The detective wavelength was set at 210 and 280 nm. Column temperature was 30 degrees C.

RESULT:

The calibration curve for atropine sulphate, diphenhydramine hydrochloride, capsaicin and dihydrocapsaicin revealed linearity in the range of 2.01-50.25, 15.08-377.00, 5.02-125.50, 5.03-125.75 mg x L(-1), respectively. The recoveries of atropine sulphate, diphenhydramine hydrochloride, capsaicin and dihydrocapsaicin were 99.00% with RSD of 0.95%, 99.89% with RSD of 1.2%, 100.1% with RSD of 1.5% and 99.51%, with RSD of 1.4%, respectively.

CONCLUSION:

The method is simple, rapid and accurate, which is suitable for the quality control of pain-relieving plaster for arthritis.

• Authors: Xiaobin Jia, Qi Liu, Yingjie Wei, Bin Chen, Yiping Chu, Jiaming Zhang
• Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (Zhongguo Zhong Yao Za Zhi) Vol. 35 Issue 21 Pg. 2838-41 (Nov 2010) ISSN: 1001-5302 [Print] China
• PMID: 21322943 (Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Drugs, Chinese Herbal
• Atropine
• Diphenhydramine
• Capsaicin
• dihydrocapsaicin

Topics

• Arthritis (drug therapy)
• Atropine (analysis, therapeutic use)
• Capsaicin (analogs & derivatives, analysis, therapeutic use)
• Chromatography, High Pressure Liquid (methods)
• Diphenhydramine (analysis, therapeutic use)
• Drugs, Chinese Herbal (analysis, therapeutic use)
• Humans
• Pain (drug therapy)