Inhibitory effects of wogonin on the invasion of human breast carcinoma cells by downregulating the expression and activity of matrix metalloproteinase-9.

Abstract	Wogonin, a naturally occurring monoflavonoid extracted from Scutellariae radix, has been shown to possess tumor therapeutic potential in vitro and in vivo. However, the effects of wogonin on tumor cells invasion remains poorly understood. In this study, we performed in vitro experiments to investigate the anti-invasive and anti-metastatic activity of wogonin in MDA-MB-231 human breast carcinoma cells. Wogonin caused a concentration-dependent suppression of cell migration, adhesion and invasion. The mechanism revealed that wogonin significantly inhibited the expression and activity of both endogenous and phorbol-12-myristate-13-acetate (PMA)-induced matrix metalloproteinase-9 (MMP-9) potentially associating with the suppression of translocation of protein kinase C (PKC) δ and phosphorylation of extracellular signal-regulated kinase (ERK1/2). These results suggested that wogonin could inhibit the invasion of tumor cells by downregulating the expression and activity of MMP-9, the possible targets may be PKCδ and ERK1/2.
Authors	Pu Chen, Na Lu, Yun Ling, Yan Chen, Hui Hui, Zhijian Lu, Xiuming Song, Zhiyu Li, Qidong You, Qinglong Guo
Journal	Toxicology (Toxicology) Vol. 282 Issue 3 Pg. 122-8 (Apr 11 2011) ISSN: 1879-3185 [Electronic] Ireland
PMID	21295103 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Copyright	Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.
Chemical References	Antineoplastic Agents, Phytogenic Flavanones Protein Kinase C-delta Extracellular Signal-Regulated MAP Kinases Matrix Metalloproteinase 9 wogonin
Topics	Antineoplastic Agents, Phytogenic (isolation & purification, pharmacology) Blotting, Western Breast Neoplasms (enzymology, pathology) Cell Adhesion (drug effects) Cell Line, Tumor Cell Movement (drug effects) Cell Survival (drug effects) Dose-Response Relationship, Drug Down-Regulation Extracellular Signal-Regulated MAP Kinases (antagonists & inhibitors) Female Flavanones (isolation & purification, pharmacology) Humans Matrix Metalloproteinase 9 (biosynthesis, metabolism) Neoplasm Invasiveness Phosphorylation Protein Kinase C-delta (antagonists & inhibitors) Scutellaria (chemistry)

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