(1→3)-β-D-Glucan inhibits a dual mechanism of peroxynitrite stroke.

The antioxidative and antinitrative activities of (1→3)-β-D-glucan (1-4μg/ml) from the yeast cell walls of Saccharomyces cerevisiae in human plasma treated with strong oxidants - peroxynitrite (ONOO(-)) (0.1mM) and hydrogen peroxide (H(2)O(2)) (2mM) were studied in vitro. The main purpose of this study was to assess if (1→3)-β-D-glucan, a well known strong immunostimulatory agent, possesses a protective function against dual mechanism of ONOO(-) stroke associated with nitrative and oxidative damages to human plasma biomolecules.
The protein changes were determined in vitro by estimating the level of oxidative stress markers - carbonyl groups, and nitrative products - 3-nitrotyrosine residues. The plasma lipid peroxidation was also investigated. The obtained results show that (1→3)-β-D-glucan inhibits in vitro ONOO(-)-induced oxidation and nitration of plasma proteins, even by 50% and 30%, respectively. The antioxidative activity of (1→3)-β-D-glucan was confirmed by its inhibitory effect on plasma lipids peroxidation induced by ONOO(-) or by H(2)O(2).
The obtained results demonstrate that (1→3)-β-D-glucan from S. cerevisiae protects plasma components against toxic effects of ONOO(-) and H(2)O(2) due to its antioxidative and antinitrative activities. Therefore (1→3)-β-D-glucan supplementation during inflammatory may be beneficial not only regard for its ability to stimulate the immune system but also by antioxidative properties.
AuthorsJoanna Saluk-Juszczak, Karolina Krolewska, Barbara Wachowicz
JournalInternational journal of biological macromolecules (Int J Biol Macromol) Vol. 48 Issue 3 Pg. 488-94 (Apr 1 2011) ISSN: 1879-0003 [Electronic] Netherlands
PMID21255603 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2011 Elsevier B.V. All rights reserved.
Chemical References
  • Antioxidants
  • Blood Proteins
  • Oxidants
  • beta-Glucans
  • Peroxynitrous Acid
  • Hydrogen Peroxide
  • Adult
  • Antioxidants (chemistry, metabolism, pharmacology)
  • Blood Proteins (drug effects)
  • Humans
  • Hydrogen Peroxide (metabolism, pharmacology)
  • Lipid Peroxidation (drug effects)
  • Male
  • Oxidants (metabolism, pharmacology)
  • Oxidation-Reduction (drug effects)
  • Oxidative Stress (drug effects)
  • Peroxynitrous Acid (metabolism, pharmacology)
  • Plasma (chemistry, drug effects)
  • Saccharomyces cerevisiae (chemistry)
  • Young Adult
  • beta-Glucans (chemistry, metabolism, pharmacology)

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