Abstract | STUDY DESIGN: Immunohistological analysis in an injured intervertebral disk (IVD) model. OBJECTIVE: To elucidate and compare in rats the behavior of the sensory nervous system and inflammatory mediators in experimentally injured IVDs. SUMMARY OF BACKGROUND DATA: Multiple human and animal studies have verified the presence of sensory nerve fibers in IVDs or investigated the behavior of inflammatory mediators in injured IVDs, but no in vivo study to date has examined the relationship between the 2. METHODS: RESULTS:
Nerve growth factor, and TNF-α levels (through 1 week) and IL-6 levels (through 4 days) were significantly higher in the disk-injured group than in the noninjured group (P < 0.05). However, starting at 2 weeks ( nerve growth factor and TNF-α) or 1 week (IL-6), the differences in inflammatory mediator levels between the 2 groups no longer were significant. In contrast, the percentage of calcitonin gene-related peptide-immunoreactive neurons among Fluoro-gold-labeled DRG neurons, and the numbers of ionized calcium-binding adaptor molecule-1-immunoreactive microglia and glial fibrillary acidic protein-immunoreactive astrocytes in the spinal dorsal horn remained significantly higher in the injured group than in the noninjured group at all-time points (P < 0.05). CONCLUSION: Disk injury in rats produces persistent increases in neuropeptides in DRGs and glia in the spinal cord, but only transient increases in inflammatory mediators in IVDs.
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Authors | Masayuki Miyagi, Tetsuhiro Ishikawa, Sumihisa Orita, Yawara Eguchi, Hiroto Kamoda, Gen Arai, Miyako Suzuki, Gen Inoue, Yasuchika Aoki, Tomoaki Toyone, Kazuhisa Takahashi, Seiji Ohtori |
Journal | Spine
(Spine (Phila Pa 1976))
Vol. 36
Issue 26
Pg. 2260-6
(Dec 15 2011)
ISSN: 1528-1159 [Electronic] United States |
PMID | 21228748
(Publication Type: Journal Article)
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Chemical References |
- Aif1 protein, rat
- Calcium-Binding Proteins
- Glial Fibrillary Acidic Protein
- Inflammation Mediators
- Interleukin-6
- Microfilament Proteins
- Neuropeptides
- Tumor Necrosis Factor-alpha
- Nerve Growth Factor
- Calcitonin Gene-Related Peptide
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Topics |
- Animals
- Calcitonin Gene-Related Peptide
(metabolism)
- Calcium-Binding Proteins
(metabolism)
- Enzyme-Linked Immunosorbent Assay
- Female
- Ganglia, Spinal
(metabolism)
- Glial Fibrillary Acidic Protein
(metabolism)
- Humans
- Immunohistochemistry
- Inflammation Mediators
(metabolism)
- Interleukin-6
(metabolism)
- Intervertebral Disc
(injuries)
- Lumbar Vertebrae
(injuries)
- Microfilament Proteins
(metabolism)
- Nerve Growth Factor
(metabolism)
- Neuroglia
(metabolism)
- Neuropeptides
(metabolism)
- Pain
(metabolism)
- Rats
- Rats, Sprague-Dawley
- Spinal Cord
(metabolism)
- Tumor Necrosis Factor-alpha
(metabolism)
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