Arachidonate 12-lipoxygenase may serve as a potential marker and therapeutic target for prostate cancer stem cells.
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| Abstract |
The presence of arachidonate 12-lipoxygenase (12-LOX) potentiates prostate cancer (PCa) progression and therefore may be a good therapeutic target and/or a potential diagnostic predictor for PCa. In this study, we examined the expression of 12-LOX in PCa stem cells (PCa SCs) to test if it can serve as a unique marker and therapeutic target for PCa SCs. To this end, we isolated the cancer stem cell-like side population (SP) cells from the human PCa cell line DU-145 by a flow cytometry-based SP technique. The isolated DU-145 SP cells comprised a small population of the DU-145 cells. The SP cells had an up-regulation of ATP-binding cassette sub-family G member 2 (ABCG2) which enables these cells to efflux vital dyes and chemotherapeutic drugs. Furthermore, we detected a strong up-regulation of 12-LOX in these DU-145 SP cells compared to the parental DU-145 cells by RT-PCR and Western blot approaches. We also detected 12-LOX overexpression in PCa SCs in human PCa tissue samples by paraffin-section based immunofluorescent 4-channel confocal microscopy. However, no 12-LOX was detected in normal prostate epithelial SCs in normal prostate tissue samples. These multiple lines of evidence support the possibility that 12-LOX may serve as a unique marker and therapeutic target for PCa SCs.
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| Authors | Bo Yin, Yang Yang, Zhiqiang Zhao, Yu Zeng, Steven M Mooney, Ming Li, Xuewen Xu, Yongsheng Song, Bin Wu, Zhibo Yang |
| Journal | International journal of oncology (Int J Oncol) Vol. 38 Issue 4 Pg. 1041-6 (Apr 2011) ISSN: 1791-2423 [Electronic] Greece |
| PMID | 21225230 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
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