Development and in vitro evaluation of deacety mycoepoxydiene nanosuspension.

Abstract

Deacety mycoepoxydiene (DM), extracted from Phomopsis sp. A123 of thalassiomycetes, is a novel and potent anti-cancer agent. Due to its physicochemical characteristics, the drug, a poorly water-soluble weak acid, shows poor solubility and dissolution characteristics. To improve the solubility and dissolution, formulation of DM as nanosuspension has been performed in this study. Nanosuspensions were developed by high-pressure homogenization (HPH) (DissoCubes(®) Technology) and transformed into dry powder by freeze-drying. The nanosuspension produced was then investigated using optical microscope, photon correlation spectroscopy (PCS), zeta potential measurement, SEM, TEM, AFM, DSC and XRD. To verify the theoretical hypothesis on the benefit of increased surface area, in vitro saturation solubility and dissolution profile were investigated. In addition, the in vitro cell cytotoxicity was examined. Results showed that a narrow size distributed nanosuspension composed of unchanged crystalline state with a mean particle size of 515±18 nm, a polydispersity index of 0.12±0.03 and a zeta potential of -23.1±3.5 mV was obtained. In the in vitro dissolution test an accelerated dissolution velocity and increased saturation solubility could be shown for the MD nanosuspension. The in vitro cytotoxicity experiments provided evidence for an enhanced efficacy of the DM nanosuspension formulation compared to free DM solution. Taken together, these results illustrate the opportunity to formulate DM in nanosuspension form as an anti-prostate cancer delivery system.

• Authors: Yancai Wang, Zhaoping Liu, Dianrui Zhang, Xihui Gao, Xiaoyu Zhang, Cunxian Duan, Lejiao Jia, Feifei Feng, Yaojian Huang, Yuemao Shen, Qiang Zhang
• Journal: Colloids and surfaces. B, Biointerfaces (Colloids Surf B Biointerfaces) Vol. 83 Issue 2 Pg. 189-97 (Apr 01 2011) ISSN: 1873-4367 [Electronic] Netherlands
• PMID: 21176876 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2010. Published by Elsevier B.V.

Chemical References

• Antineoplastic Agents
• Epoxy Compounds
• Pyrones
• deacety mycoepoxydiene

Topics

• Antineoplastic Agents (chemical synthesis, chemistry, pharmacology)
• Cell Proliferation (drug effects)
• Crystallography, X-Ray
• Drug Screening Assays, Antitumor
• Epoxy Compounds (chemical synthesis, chemistry, pharmacology)
• Humans
• Male
• Molecular Conformation
• Nanostructures (chemistry)
• Particle Size
• Pyrones (chemical synthesis, chemistry, pharmacology)
• Solubility
• Tumor Cells, Cultured