Abstract | AIMS: METHODS AND RESULTS: Immunohistochemistry of normal and cancer tissues was performed utilizing three commercial polyclonal antibodies: anti-methylarginine-specific antibody (anti-mRG) raised against a methylarginine peptide, Control antibody (anti-RG), a control antiserum raised against a corresponding arginine peptide without any methylated residues and anti-PRMT1. Nuclear and/or cytoplasmic methylarginine expression was detected in all keratinized and non-keratinized epithelia. A preliminary survey of a series of thyroid, pancreatic, colonic and gastric cancers identified a different pattern of methylarginine expression in comparison with normal tissue. A correlation between methylarginine staining and PRMT1 expression was found in all normal and cancer tissues analysed. CONCLUSION: Methylarginine-specific antibodies are capable of recognizing methylarginine proteins (MeRP) in paraffin-embedded tissues. Methylarginine proteins are expressed widely and show differences in subcellular localization in various organs and neoplastic conditions. The efficient detection of methylproteins by standard immunohistochemistry provides a new tool to investigate the role of methylarginine proteins (MeRP) in biological processes including carcinogenesis.
|
Authors | Marzia Vezzalini, John M Aletta, Stefania Beghelli, Elisabetta Moratti, Marco Della Peruta, Andrea Mafficini, Wilfrido D Mojica, Aldo Mombello, Aldo Scarpa, Claudio Sorio |
Journal | Histopathology
(Histopathology)
Vol. 57
Issue 5
Pg. 725-33
(Nov 2010)
ISSN: 1365-2559 [Electronic] England |
PMID | 21083602
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | © 2010 Blackwell Publishing Limited. |
Chemical References |
- Antibodies
- Proteins
- Arginine
- Protein-Arginine N-Methyltransferases
|
Topics |
- Antibodies
(immunology)
- Antibody Specificity
- Arginine
(immunology, metabolism)
- Immunohistochemistry
- Methylation
- Neoplasms
(metabolism, pathology)
- Paraffin Embedding
- Protein Processing, Post-Translational
- Protein-Arginine N-Methyltransferases
(metabolism)
- Proteins
(metabolism)
|