Proteomic analysis of human gastric juice: a shotgun approach.

Abstract

Gastric juice is the most proximal fluid surrounding the stomach tissue. The analysis of gastric juice protein contents will thus be able to accurately reflect the pathophysiology of the stomach. This biological fluid is also a potential reservoir of secreted biomarkers in higher concentration as compared to the serum. Unlike the rest of the gastrointestinal fluids, there were very few studies reported on gastric juice proteome. To date, the proteins that routinely populate this biofluid are largely unknown. This is partly due to the technical difficulties in processing a sample that contains a collection of other gastrointestinal fluids, especially saliva. In this study, we attempt to profile the protein components of the gastric fluids from chronic gastritis patients using a direct shotgun proteomics approach. These data represent the first report of the proteome of human gastric juice with gastritis background.

• Authors: Cynthia R M Y Liang, Sandra Tan, Hwee Tong Tan, Qingsong Lin, Teck Kwang Lim, Yi Liu, Khay Guan Yeoh, Jimmy So, Maxey C M Chung
• Journal: Proteomics (Proteomics) Vol. 10 Issue 21 Pg. 3928-31 (Nov 2010) ISSN: 1615-9861 [Electronic] Germany
• PMID: 20925057 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Proteins

Topics

• Aged
• Aged, 80 and over
• Chronic Disease
• Databases, Protein
• Female
• Gastric Juice (chemistry)
• Gastritis (metabolism)
• Humans
• Male
• Middle Aged
• Peptide Mapping (methods)
• Proteins (analysis, chemistry, classification, genetics)
• Proteomics (methods)