[Protective effects of asiaticoside on sepsis-induced acute kidney injury in mice].

Abstract

OBJECTIVE:

The present study is to investigate the protective effects of asiaticoside on sepsis-induced acute kidney injury in mice.

METHOD:

With the sepsis induced by cecal ligation and puncture (CLP), forty eight kunming mice were randomly divided into four groups as sham operated, CLP treated, CLP + asiaticoside 15, 45 mg x kg(-1) groups. General conditions and the amount of dead rate of mice were observed. The BUN and Cr levels were observed by the kits. IL-6 in serum was assayed by enzyme-linked immunosorbent assay (ELISA). Kidney tissues were harvested for determination of iNOS expression by Western blotting analysis. The pathologic changes were observed under electron microscope via hematoxylin-eosin (HE) stain.

RESULT:

Compared with CLP group, the death rate, the levels of BUN, Cr, IL-6, and iNOS protein expression of asiaticoside groups were significantly reduced. The pathologic changes in kidney tissues induced by sepsis were significantly attenuated dose-dependently by asiaticoside under electron microscope.

CONCLUSION:

Asiaticoside has protective effects against sepsis-induced acute kidney injury, which were probably associated with the inhibition of IL-6 in serum and iNOS protein in kidney tissues.

• Authors: Jiajia Zheng, Li'na Zhang, Mengjiao Wu, Xiaohui Li, Li Zhang, Jingyuan Wan
• Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (Zhongguo Zhong Yao Za Zhi) Vol. 35 Issue 11 Pg. 1482-5 (Jun 2010) ISSN: 1001-5302 [Print] China
• PMID: 20822026 (Publication Type: English Abstract, Journal Article)

Chemical References

• Plant Extracts
• Triterpenes
• asiaticoside

Topics

• Acute Kidney Injury (drug therapy, etiology, prevention & control)
• Animals
• Disease Models, Animal
• Female
• Humans
• Male
• Mice
• Plant Extracts (administration & dosage)
• Random Allocation
• Sepsis (complications)
• Triterpenes (administration & dosage)