Inhibitory effects of 1-O-methyl-fructofuranose from Schisandra chinensis fruit on melanogenesis in B16F0 melanoma cells.

Abstract

AIM OF THE STUDY:

1-O-methyl-fructofuranose (1-O-MFF) from the fruit of Schisandra chinensis is a traditional Korean medicinal herb that has a variety of beneficial properties. The effect of purified 1-O-MFF on melanogenesis including the activation of related signaling pathways was investigated.

MATERIALS AND METHODS:

The inhibitory activities of 1-O-MFF were examined by melanin synthesis, tyrosinase activity assay, Western blot and flow cytometric analyses in B16F0 mouse melanoma cells.

RESULTS:

1-O-MFF significantly inhibited both melanin synthesis and tyrosinase activity in a concentration-dependent manner, and reduced the expression of melanogenic proteins including microphthalmia-associated transcription factor (MITF), tyrosinase and tyrosinase-related protein-1. 1-O-MFF phosphorylated and activated melanogenesis inhibitory proteins such as mitogen-activated protein kinase kinase (MEK)/extracellular signal-regulated kinase (ERK) and Akt. Flow cytometry confirmed that 1-O-MFF phosphorylated ERK and Akt proteins and recovered partially phosphorylated forms in cells treated with the MEK/ERK inhibitor compound PD98059 and the phosphatidylinositol 3-kinase (PI3K)/Akt inhibitor compound LY294002.

CONCLUSIONS:

The suppressive effects of 1-O-MFF on melanogenesis may involve down-regulation of MITF and its downstream signal pathway via the activation of MEK/ERK or PI3K/Akt.

• Authors: Eun Young Oh, Ji Yeon Jang, Yung Hyun Choi, Young Whan Choi, Byung Tae Choi
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 132 Issue 1 Pg. 219-24 (Oct 28 2010) ISSN: 1872-7573 [Electronic] Ireland
• PMID: 20723590 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2010 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Melanins
• Microphthalmia-Associated Transcription Factor
• Mitf protein, mouse
• methylfructoside
• Fructose
• Monophenol Monooxygenase
• Phosphatidylinositol 3-Kinases
• Extracellular Signal-Regulated MAP Kinases
• Mitogen-Activated Protein Kinase Kinases

Topics

• Animals
• Blotting, Western
• Cell Line, Tumor
• Cell Survival (drug effects)
• Extracellular Signal-Regulated MAP Kinases (metabolism)
• Flow Cytometry
• Fructose (analogs & derivatives, isolation & purification, pharmacology)
• Fruit (chemistry)
• Medicine, Korean Traditional
• Melanins (antagonists & inhibitors, biosynthesis)
• Melanoma, Experimental (enzymology, metabolism, pathology)
• Mice
• Microphthalmia-Associated Transcription Factor (metabolism)
• Mitogen-Activated Protein Kinase Kinases (metabolism)
• Monophenol Monooxygenase (metabolism)
• Phosphatidylinositol 3-Kinases (metabolism)
• Schisandra (chemistry)