Time-dependent changes in GLT-1 functioning in striatum of hemi-Parkinson rats.

Abstract

Striatal dopamine loss in Parkinson's disease is accompanied by a dysregulation of corticostriatal glutamatergic neurotransmission. Within this study, we investigated striatal expression and activity of the glial high-affinity Na(+)/K(+)-dependent glutamate transporters, GLT-1 and GLAST, in the 6-hydroxydopamine hemi-Parkinson rat model at different time points after unilateral 6-hydroxydopamine injection into the medial forebrain bundle. Using semi-quantitative Western blotting and an ex vivo D-[(3)H]-aspartate uptake assay, we showed a time-dependent bilateral effect of unilateral 6-hydroxydopamine lesioning on the expression as well as activity of GLT-1. At 3 and 12 weeks post-lesion, striatal GLT-1 function was bilaterally upregulated whereas at 5 weeks there was no change. Even though our data do not allow a straightforward conclusion as for the role of glutamate transporters in the pathogenesis of the disease, they do clearly demonstrate a link between disturbed glutamatergic neurotransmission and glutamate transporter functioning in the striatum of a rat model for Parkinson's disease.

• Authors: Ann Massie, Stéphanie Goursaud, Anneleen Schallier, Katia Vermoesen, Charles K Meshul, Emmanuel Hermans, Yvette Michotte
• Journal: Neurochemistry international (Neurochem Int) Vol. 57 Issue 5 Pg. 572-8 (Nov 2010) ISSN: 1872-9754 [Electronic] England
• PMID: 20643175 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2010 Elsevier Ltd. All rights reserved.

Chemical References

• Excitatory Amino Acid Transporter 1
• Excitatory Amino Acid Transporter 2
• Piperazines
• Slc1a2 protein, rat
• Slc1a3 protein, rat
• Aspartic Acid
• Oxidopamine
• 1-(2 (diphenylmethoxy)ethyl)-4-(3-phenylpropyl)piperazine
• Dopamine

Topics

• Animals
• Aspartic Acid (metabolism)
• Blotting, Western
• Chromatography, High Pressure Liquid
• Corpus Striatum (metabolism, physiology)
• Dopamine (metabolism)
• Excitatory Amino Acid Transporter 1 (biosynthesis, genetics)
• Excitatory Amino Acid Transporter 2 (biosynthesis, genetics, physiology)
• Immunohistochemistry
• Medial Forebrain Bundle (metabolism, physiology)
• Oxidopamine
• Parkinson Disease, Secondary (chemically induced, physiopathology)
• Piperazines (metabolism)
• Rats
• Synaptosomes (metabolism)
• Time Factors