Abstract |
Methanohalophilus portucalensis FDF1 can grow over a range of external NaCl concentrations, from 1.2 to 2.9 mol/L. Differential gene expression in response to long-term hyper-salt stress (3.1 mol/L of NaCl) and hypo-salt stress (0.9 mol/L of NaCl) were compared by differential display RT-PCR. Fourteen differentially expressed genes responding to long-term hyper- or hypo-salt stress were detected, cloned, and sequenced. Several of the differentially expressed genes were related to the unique energy-acquiring methanogenesis pathway in this organism, including the transmembrane protein MttP, cobalamin biosynthesis protein, methenyl-H4MPT cyclohydrolase and monomethylamine methyltransferase. One signal transduction histidine kinase was identified from the hyper-salt stress cultures. Moreover, 3 known stress-response gene homologues - the DNA mismatch repair protein, MutS, the universal stress protein, UspA, and a member of the protein-disaggregating multichaperone system, ClpB - were also detected. The transcriptional analysis of these long-term salt stress response and adaptation-related genes for cells immediately after salt stress indicated that the expression of the energy metabolism genes was arrested during hyper- salt shock, while the chaperone clpB gene was stimulated by both hypo- and hyper- salt shock.
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Authors | Chao-Jen Shih, Mei-Chin Lai |
Journal | Canadian journal of microbiology
(Can J Microbiol)
Vol. 56
Issue 4
Pg. 295-307
(Apr 2010)
ISSN: 1480-3275 [Electronic] Canada |
PMID | 20453896
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Adaptation, Physiological
- Base Sequence
- Energy Metabolism
- Gene Expression Profiling
- Gene Expression Regulation, Archaeal
- Methanosarcinaceae
(genetics, metabolism)
- Molecular Sequence Data
- Reverse Transcriptase Polymerase Chain Reaction
- Sodium Chloride
(metabolism)
- Stress, Physiological
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