Abstract | BACKGROUND: RESULTS: Using both EpCAM antibodies ( mAb BerEP4 and mAb KS1/4) for immunomagnetic enrichment in blood samples of 39 patients with colorectal cancer we found heterogenous results in each patient with regard to tumor cell detection. In the tumor cell spiking experiments with whole blood samples the sensitivity of the CK 20 RT-PCR assay was higher using immunomagnetic beads coated with mAb KS1/4 compared to precoated mAb BerEP4 Dynabeads. Extraction of MNC fraction with Ficoll gradient centrifugation prior to immunomagnetic enrichment resulted in a higher sensitivity of the CK 20 RT-PCR assay. CONCLUSIONS: We concluded that isolation and detection of CTC with immunomagnetic enrichment methods is critically dependent on the used EpCAM clone. Further studies with a larger number of patients should clarify if the enrichment protocol influences the prognostic value of the tumor cell detection protocol.
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Authors | Dalibor Antolovic, Luis Galindo, Anina Carstens, Nuh Rahbari, Markus W Büchler, Jürgen Weitz, Moritz Koch |
Journal | BMC biotechnology
(BMC Biotechnol)
Vol. 10
Pg. 35
(Apr 28 2010)
ISSN: 1472-6750 [Electronic] England |
PMID | 20426872
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Antibodies, Neoplasm
- Antigens, Neoplasm
- Cell Adhesion Molecules
- Epithelial Cell Adhesion Molecule
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Topics |
- Adult
- Aged
- Aged, 80 and over
- Antibodies, Neoplasm
(chemistry)
- Antigens, Neoplasm
(isolation & purification)
- Cell Adhesion Molecules
(isolation & purification)
- Colorectal Neoplasms
(blood, diagnosis)
- Epithelial Cell Adhesion Molecule
- Female
- HT29 Cells
- Humans
- Immunomagnetic Separation
- Male
- Middle Aged
- Neoplastic Cells, Circulating
- Sensitivity and Specificity
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