Detection of indoor and outdoor avian antigen in management of bird-related hypersensitivity pneumonitis.

Abstract

BACKGROUND:

In the management of hypersensitivity pneumonitis (HP), antigen avoidance is crucial to prevent the progression of disease. Indirect and unrecognized exposure to the antigen may continue for a long time if persistence of the causative antigen is not recognized. To make a correct assessment of the patients' environment, we tried to establish the methods to detect indoor and outdoor avian antigens.

METHODS:

Sixteen patients with bird-related HP, 4 asymptomatic breeders, and 6 healthy controls were examined. We prepared anti-pigeon dropping extracts (PDE) polyclonal antibody from rabbits. Air samples and house dust samples were analyzed by an antigen-capture ELISA with signal amplification using catalyzed reporter deposition.

RESULTS:

In air samples, avian antigen could be detected in patients with HP (0.73 +/- 0.53 ng/m3) and asymptomatic breeders (0.63 +/- 0.23 ng/m3). In house dust samples, the amount of avian antigen was higher in patients with HP (2.4 +/- 1.8 microg/g) and asymptomatic breeders (4.1 +/- 2.3 microg/g) than in the controls (0.1 +/- 0.2 microg/g).

CONCLUSIONS:

Detection of indoor and outdoor avian antigen might contribute to the correct diagnosis and appropriate managements of bird-related HP.

• Authors: Jin Kuramochi, Naohiko Inase, Koji Takayama, Yasunari Miyazaki, Yasuyuki Yoshizawa
• Journal: Allergology international : official journal of the Japanese Society of Allergology (Allergol Int) Vol. 59 Issue 2 Pg. 223-8 (Jun 2010) ISSN: 1440-1592 [Electronic] England
• PMID: 20414051 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Avian Proteins
• Complex Mixtures
• Dust

Topics

• Air (analysis)
• Air Pollution, Indoor (adverse effects)
• Alveolitis, Extrinsic Allergic (diagnosis, epidemiology, immunology, therapy)
• Animals
• Avian Proteins (chemistry, immunology)
• Columbidae (immunology)
• Complex Mixtures
• Dust (analysis)
• Environmental Exposure (adverse effects)
• Enzyme-Linked Immunosorbent Assay
• Feasibility Studies
• Humans
• Immunologic Tests (methods)
• Rabbits