Agaritine purified from Agaricus blazei Murrill exerts anti-tumor activity against leukemic cells.

Abstract

BACKGROUND:

Mushrooms of the genus Agaricus are a common folk remedy against carcinoma. The active ingredients, polysaccharides and protein-polysaccharide complexes containing beta-glucan, have been isolated and shown to have indirect tumor-suppressing activity via an immunological activation.

METHODS:

The diffusible fraction of a hot-water extract of Agaricus blazei Murrill (ABM) powder was fractionated by HPLC based on the anti-tumor activity against leukemic cells in vitro. The structure of the anti-tumor substance was determined by NMR and MS analyses.

RESULTS:

We purified a tumorcidal substance from the diffusible fraction of ABM and identified it as agaritine, beta-N-(gamma-l(+)-glutamyl)-4-(hydroxymethyl) phenylhydrazine, having a molecular mass of 267 Da. This compound inhibited the proliferation of leukemic cell lines such as U937, MOLT4, HL60 and K562 with IC(50) values of 2.7, 9.4, 13.0, and 16.0 microg/mL, respectively, but showed no significant effect on normal lymphatic cells at concentrations up to 40 microg/mL. Although agaritine has been suspected of having genotoxic or carcinogenic properties, agaritine did not activate the umu gene of Salmonella, which reacts to carcinogens.

GENERAL SIGNIFICANCE:

The results indicate that agaritine from ABM has direct anti-tumor activity against leukemic tumor cells in vitro. This is in contrast to the carcinogenic activity previously ascribed to this compound. Our results also show that this activity is distinct from that of beta-glucan, which indirectly suppresses proliferation of tumor cells.

• Authors: Masahiro Endo, Hidehiko Beppu, Hidehiko Akiyama, Kazumasa Wakamatsu, Shosuke Ito, Yasuko Kawamoto, Kan Shimpo, Toshimitu Sumiya, Takaaki Koike, Taei Matsui
• Journal: Biochimica et biophysica acta (Biochim Biophys Acta) Vol. 1800 Issue 7 Pg. 669-73 (Jul 2010) ISSN: 0006-3002 [Print] Netherlands
• PMID: 20347942 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2010 Elsevier B.V. All rights reserved.

Chemical References

• Antineoplastic Agents
• Phenylhydrazines
• agaritine

Topics

• Agaricus (chemistry)
• Antineoplastic Agents (pharmacology)
• Cell Line, Tumor (drug effects)
• Cell Survival (drug effects)
• Chromatography, High Pressure Liquid (methods)
• HL-60 Cells (drug effects)
• Humans
• K562 Cells (drug effects)
• Lymphocytes (drug effects)
• Models, Molecular
• Phenylhydrazines (chemistry, isolation & purification, pharmacology)
• U937 Cells (drug effects)