In vitro and in vivo evaluation of docetaxel loaded biodegradable polymersomes.

Abstract

Formulation of docetaxel (DOC), a hydrophobic anticancer drug, was successfully achieved in poly(gamma-benzyl L-glutamate)-block-hyaluronan polymersomes using a simple and reproducible nanoprecipitation method. The prepared DOC loaded polymersomes (PolyDOC) was stable either in solution or in a lyophilized form, and showed controlled release behaviour over several days. PolyDOC showed high in vitro toxicity after 24 h in MCF-7 and U87 cells compared to free DOC. Biodistribution data demonstrated that (99m)Tc labelled PolyDOC t(1/2) and MRT significantly increased compared to a DOC solution (DS). In addition, PolyDOC uptake in Ehrlich Ascites Tumor (EAT) tumor bearing mice was larger at each time point compared to DS, making such a polymer vesicle formulation an efficient drug nanocarrier for improved DOC cancer therapy.

• Authors: Kamal K Upadhyay, Anant N Bhatt, Emilio Castro, Anil K Mishra, Krishna Chuttani, Bilikere S Dwarakanath, Christophe Schatz, Jean-François Le Meins, Ambikanandan Misra, Sébastien Lecommandoux
• Journal: Macromolecular bioscience (Macromol Biosci) Vol. 10 Issue 5 Pg. 503-12 (May 14 2010) ISSN: 1616-5195 [Electronic] Germany
• PMID: 20232310 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Capsules
• Delayed-Action Preparations
• Taxoids
• poly(gamma-benzyl glutamate)-b-hyaluronan
• Docetaxel
• Polyglutamic Acid
• Hyaluronic Acid

Topics

• Animals
• Antineoplastic Agents (administration & dosage, pharmacokinetics)
• Capsules
• Cell Line, Tumor
• Delayed-Action Preparations (chemistry)
• Docetaxel
• Female
• Humans
• Hyaluronic Acid (analogs & derivatives, chemistry)
• Mice
• Mice, Inbred BALB C
• Polyglutamic Acid (analogs & derivatives, chemistry)
• Rabbits
• Taxoids (administration & dosage, pharmacokinetics)