Microarray analysis of tonsils in immunoglobulin A nephropathy patients.

Abstract

BACKGROUND:

Recently, combination of tonsillectomy and steroid pulse therapy was reported to be effective as the treatment of the immunoglobulin A nephropathy (IgAN). However, the gene expression difference between the tonsils in patients with IgAN and those in control patients is not established.

METHODS:

We performed tonsillectomy combined with steroid pulse as a treatment to IgAN, analyzed the gene expression in the tonsils (N=23) using microarray, compared with those with patients suffering from chronic tonsillitis (N=22). From some candidate genes related with IgAN, we confirmed the apolipoprotein B messenger RNA-editing enzyme catalytic polypeptides 2 (APOBEC2) gene expression in the tonsil and we also analyzed its expression levels and clinical features.

RESULTS:

Up-regulated genes seem to be categorized into two groups. One group belongs to the muscle related genes which might be caused by structural differences. The other group includes the immune system-related genes, such as APOBEC2, CALB2, DUSP27, and CXCL11. APOBEC2 was positively stained in the epithelium and the peripheral region of the germinal center in both tonsils. APOBEC2 expression level was negatively related with serum igg level, but did not correlate with clinical course after tonsillectomy.

CONCLUSION:

We confirmed gene expression differences related with immune system and muscle structure. The APOBEC2 was confirmed to be elevated in the tonsils with IgAN patients, and the gene expression level was negatively related with serum igg level in overall patients. These results might be helpful to reveal the mechanism of IgAN.

• Authors: Kenichiro Iio, Yasuyuki Nagasawa, Hirotsugu Iwatani, Ryohei Yamamoto, Arata Horii, Daisuke Okuzaki, Yoshiyuki Furumatsu, Hidenori Inohara, Hiroshi Nojima, Enyu Imai, Yoshitaka Isaka, Hiromi Rakugi
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 393 Issue 4 Pg. 565-70 (Mar 19 2010) ISSN: 1090-2104 [Electronic] United States
• PMID: 20138841 (Publication Type: Journal Article)
• Copyright: Copyright 2010 Elsevier Inc. All rights reserved.

Chemical References

• Adrenal Cortex Hormones
• Immunoglobulin G
• Muscle Proteins
• APOBEC Deaminases
• APOBEC2 protein, human
• Cytidine Deaminase

Topics

• APOBEC Deaminases
• Adrenal Cortex Hormones (administration & dosage)
• Adult
• Cytidine Deaminase (genetics)
• Down-Regulation
• Female
• Gene Expression Profiling
• Glomerulonephritis, IGA (genetics, immunology, therapy)
• Humans
• Immunoglobulin G (blood)
• Male
• Middle Aged
• Muscle Proteins (genetics)
• Oligonucleotide Array Sequence Analysis
• Palatine Tonsil (immunology, pathology)
• Pulse Therapy, Drug
• Tonsillectomy
• Tonsillitis (genetics, immunology, surgery)
• Up-Regulation
• Young Adult