Rapid diagnosis in early stage renal tuberculosis by real-time polymerase chain reaction on renal biopsy specimens.

Abstract

OBJECTIVE:

To determine the early rapid diagnosis of renal tuberculosis (RTB) by real-time polymerase chain reaction (PCR) on renal biopsy specimens.

METHODS:

Ninety patients were selected for this study. The patients were divided into the following three groups: RTB, non-RTB (N-RTB) and clinically suspected RTB (CS-RTB). The renal biopsy specimens of these patients were used for Mycobacterium tuberculosis DNA detection by real-time PCR, using 35 and 40 as cycle threshold (C(T)) cut-off values. Morning urine samples were collected for M. tuberculosis culture.

RESULTS:

In the RTB group, 25 C(T)35 and 28 C(T)40 patients were PCR-positive, seven of whom were urine M. tuberculosis culture-positive. In the N-RTB group, four C(T)35 and 13 C(T)40 patients were PCR-positive, none of whom were urine M. tuberculosis culture-positive. In the CS-RTB group, nine C(T)35 and 14 C(T)40 patients were PCR-positive, two of whom were urine M. tuberculosis culture-positive during 12 months of follow-up. The sensitivity and specificity of real-time PCR (C(T)40) were respectively 93.3% and 56.7%. The sensitivity and specificity of real-time PCR (C(T)35) were respectively 83.3% and 86.7%. The sensitivity and specificity of the urine M. tuberculosis culture were respectively 23.3% and 100%.

CONCLUSIONS:

The detection of M. tuberculosis DNA in renal biopsy tissue by real-time PCR is highly sensitive. Real-time PCR can increase diagnostic accuracy and provide valuable information regarding the early diagnosis of RTB.

• Authors: L Sun, Q Yuan, J-M Feng, C-M Yang, L Yao, Q-L Fan, L-L Liu, J-F Ma, L-N Wang
• Journal: The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease (Int J Tuberc Lung Dis) Vol. 14 Issue 3 Pg. 341-6 (Mar 2010) ISSN: 1815-7920 [Electronic] France
• PMID: 20132626 (Publication Type: Journal Article)

Chemical References

• DNA, Bacterial

Topics

• Adult
• Biopsy (methods)
• DNA, Bacterial (analysis)
• Female
• Follow-Up Studies
• Humans
• Male
• Middle Aged
• Mycobacterium tuberculosis (isolation & purification)
• Polymerase Chain Reaction (methods)
• Reproducibility of Results
• Sensitivity and Specificity
• Tuberculosis, Renal (diagnosis, microbiology)
• Young Adult