Caveolae-mediated entry of Salmonella typhimurium into senescent nonphagocytotic host cells.

Abstract

Elderly individuals have an increased susceptibility to microbial infections because of age-related anatomical, physiological, and environmental factors. However, the mechanism of aging-dependent susceptibility to infection is not fully understood. Here, we found that caveolae-dependent endocytosis is elevated in senescent cells. Thus, we focused on the implications of caveolae-dependent endocytosis using Salmonella typhimurium, which causes a variety of diseases in humans and animals by invading the eukaryotic host cell. Salmonella invasion increased in nonphagocytotic senescent host cells in which caveolin-1 was also increased. When caveolae structures were disrupted by methyl-beta-cyclodextrin or siRNA of caveolin-1 in the senescent cells, Salmonellae invasion was reduced markedly compared to that in nonsenescent cells. In contrast, the over-expression of caveolin-1 led to increased Salmonellae invasion in nonsenescent cells. Moreover, in aged mice, caveolin-1 was found to be highly expressed in Peyer's patch and spleen, which are targets for infection by Salmonellae. These results suggest that high levels of caveolae and caveolin-1 in senescent host cells might be related to the increased susceptibility of elderly individuals to microbial infections.

• Authors: Jae Sung Lim, Hyon E Choy, Sang Chul Park, Jung Min Han, Ik-Soon Jang, Kyung A Cho
• Journal: Aging cell (Aging Cell) Vol. 9 Issue 2 Pg. 243-51 (Apr 2010) ISSN: 1474-9726 [Electronic] England
• PMID: 20096033 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Caveolin 1

Topics

• Adolescent
• Aged
• Aged, 80 and over
• Animals
• Caveolae (physiology)
• Caveolin 1 (genetics, metabolism)
• Cells, Cultured
• Cellular Senescence
• Gene Expression Regulation
• Humans
• Mice
• Mice, Inbred C57BL
• Salmonella typhimurium (physiology)