(-)-Nyasol (cis-hinokiresinol), a norneolignan from the rhizomes of Anemarrhena asphodeloides, is a broad spectrum inhibitor of eicosanoid and nitric oxide production.

Abstract

To assess the anti-inflammatory activity of constituents from the rhizomes of Anemarrhena asphodeloides, (-)-nyasol {cis-hinokiresinol, 4,4-[1Z,3R]-3-ethenyl-1-propene-1,3-diyl]bisphenol} was isolated and its anti-inflammatory activity was examined in lipopolysaccharide (LPS)-treated RAW 264.7 cells and A23187-treated RBL-1 cells. In vivo activity was measured using carrageenan-induced paw edema assay. At > 1 microM, (-)-nyasol significantly inhibited cyclooxygenase-2 (COX-2)-mediated PGE2 production and inducible nitric oxide synthase (iNOS)-mediated NO production in LPS-treated RAW 264.7 cells, a mouse macrophage-like cell line, but did not affect the expression levels of COX-2 and iNOS. (-)-Nyasol also inhibited 5-lipoxygenase (5-LOX)-mediated leukotriene production in A23187-treated RBL-1 cells. Furthermore, (-)-nyasol potently inhibited carrageenan-induced paw edema in mice (28.6-77.1% inhibition at 24-120 mg/kg). Therefore, (-)-nyasol is a potential new lead compound and may contribute to the anti-inflammatory action of A. asphodeloides, possibly by inhibiting COX-2, iNOS and 5-LOX.

• Authors: Hyun Lim, Joo Won Nam, Eun-Kyoung Seo, Yeong Shik Kim, Hyun Pyo Kim
• Journal: Archives of pharmacal research (Arch Pharm Res) Vol. 32 Issue 11 Pg. 1509-14 (Nov 2009) ISSN: 0253-6269 [Print] Korea (South)
• PMID: 20091263 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents
• Cyclooxygenase 2 Inhibitors
• Eicosanoids
• Lignans
• Lipopolysaccharides
• Lipoxygenase Inhibitors
• Phenols
• Nitric Oxide
• nyasol
• Nitric Oxide Synthase Type II
• Cyclooxygenase 2

Topics

• Anemarrhena (chemistry)
• Animals
• Anti-Inflammatory Agents (administration & dosage, isolation & purification, pharmacology)
• Cyclooxygenase 2 (drug effects, metabolism)
• Cyclooxygenase 2 Inhibitors (administration & dosage, isolation & purification, pharmacology)
• Disease Models, Animal
• Dose-Response Relationship, Drug
• Eicosanoids (biosynthesis)
• Inflammation (drug therapy, physiopathology)
• Lignans (administration & dosage, isolation & purification, pharmacology)
• Lipopolysaccharides
• Lipoxygenase Inhibitors
• Male
• Mice
• Mice, Inbred ICR
• Nitric Oxide (biosynthesis)
• Nitric Oxide Synthase Type II (antagonists & inhibitors)
• Phenols (administration & dosage, isolation & purification, pharmacology)
• Rats
• Rhizome