Effect of diabetes mellitus on acinar morphology, peroxidase concentration, and release in isolated rat lacrimal glands.
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| Abstract | PURPOSE: This study investigated the effect of diabetes mellitus on lacrimal gland morphology and function. MATERIALS AND METHODS: The lacrimal glands of rats (n = 6) were removed 8 weeks after the onset of diabetes and processed for electron microscopy. The lacrimal gland of control rats (n = 6) were processed similarly. Lacrimal tissue samples of diabetic rats (n = 12) were also incubated with different concentrations (10(-6)-10(-3) M) of acetylcholine and noradrenaline to investigate secretagogue-induced peroxidase release. The lacrimal glands of control rats (n = 12) were treated in a similar manner. RESULTS: Diabetic rats and their lacrimal glands gained significantly (p < 0.05) less weight compared to age-matched controls. Lacrimal acinar cells of diabetic rat have significantly (p < 0.001) smaller and more homogenous secretory granules compared to age-matched control. Lacrimal glands of diabetic rats contained significantly (p < 0.05) less peroxidase and secrete significantly less quantity (p < 0.05) of the enzyme in response to either acetylcholine or noradrenaline challenge compared to control glands. CONCLUSIONS: The results indicate that diabetes is associated with lacrimal gland insufficiency as a result of abnormal acinar morphology and reduced peroxidase content and secretion.
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| Authors | Raksha Shetty, Tariq Saeed, Hameed Rashed, Ernest Adeghate, Jaipaul Singh |
| Journal | Current eye research (Curr Eye Res) Vol. 34 Issue 10 Pg. 905-11 (Oct 2009) ISSN: 1460-2202 [Electronic] England |
| PMID | 19895318 (Publication Type: Journal Article) |
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