Association of TNFA (-308G>A) and IL-10 (-819C>T) promoter polymorphisms with risk of cervical cancer.

Abstract

Etiology of cervical cancer is associated with excessive inflammation mediated tumorigenesis. Pro and anti-inflammatory cytokines (tumor necrosis factor alpha, TNFA and interleukin, IL-10) are involved in fighting against the tumorigenesis. Therefore, the present study was designed to evaluate the association of TNFA (-308G>A) and IL-10 (-819C>T) gene polymorphism with risk of cervical cancer. One hundred fifty histopathologically confirmed patients with cervical cancer and 162 age, ethnically-matched cervical cytology negative healthy controls were genotyped for TNFA (-308 G>A) and IL-10 (-819 C>T) polymorphisms using PCR-RFLP. Individuals with combination of TNFA -308GA+AA genotype and A allele were at elevated risk of cervical cancer (odds ratio (OR) = 2.24; P = 0.018 and OR, 2.05; P = 0.012). Frequency of IL-10 -819CT+TT genotype combination and T allele was slightly higher in cases as compared with controls but difference was not significant (OR = 1.52; P = 0.069 and OR = 1.38; P = 0.051). In association of genotypes with clinical characteristics, presence of TNFA -308GA+AA genotype conferred high risk for the stages (IB) (OR = 2.86, P = 0.039) and stages (III) (OR = 2.52; P = 0.015) of cervical cancer. In contrast, IL-10 -819TT genotype was not associated with higher risk of clinical characteristics of cervical cancer. In conclusion, individuals with TNFA -308*A allele carriers were at significantly higher risk of cervical cancer particularly early (IB) and advanced stages (III). However, IL-10 (-819C>T) polymorphism was not associated with risk of cervical cancer.

• Authors: HariOm Singh, Meenu Jain, Rekha Sachan, Balraj Mittal
• Journal: International journal of gynecological cancer : official journal of the International Gynecological Cancer Society (Int J Gynecol Cancer) Vol. 19 Issue 7 Pg. 1190-4 (Oct 2009) ISSN: 1525-1438 [Electronic] England
• PMID: 19823053 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• IL10 protein, human
• Tumor Necrosis Factor-alpha
• Interleukin-10

Topics

• Adult
• Carcinoma (genetics, pathology)
• DNA Mutational Analysis
• Environment
• Female
• Genetic Linkage
• Genetic Predisposition to Disease
• Humans
• Interleukin-10 (genetics)
• Middle Aged
• Neoplasm Staging
• Polymorphism, Restriction Fragment Length
• Polymorphism, Single Nucleotide
• Promoter Regions, Genetic
• Risk Factors
• Tumor Necrosis Factor-alpha (genetics)
• Uterine Cervical Neoplasms (genetics, pathology)