Mustard oil in "Shibori Daikon" a variety of Japanese radish, selectively inhibits the proliferation of H-ras-transformed 3Y1 cells.

Abstract

Cruciferous vegetables and their isothiocyanates are promising foods and agents for cancer prevention. We focus here on the effects of mustard oil (SMO) in a variety of the Japanese radish, Shibori Daikon (Raphanus sativus), on the proliferation of 3Y1 rat fibroblasts and the H-ras-transformed derivative, HR-3Y1-2. SMO (1.6 microg/ml) inhibited the proliferation of HR-3Y1-2, but not 3Y1 after 24 h after treatment. A cell cycle analysis showed that SMO induced G2/M arrest after 6 h, although this effect was not observed 24 h after the treatment. SMO transiently decreased the cellular reduced glutathione level accompanied with up-regulation of the intracellular reactive oxygen species 2-3 h post-treatment. Glutathione ethyl ester and N-acetyl-L-cysteine prevented the growth inhibitory effect of SMO. This mustard oil extract consisted of 95.6% 4-methylthio-3-butenyl isothiocyanate and 4.4% 4-methylthiobutyl isothiocyanate. SMO selectively inhibited H-ras-transformed 3Y1 cells associated with transient oxidative stress via reduced glutathione (GSH) depletion.

• Authors: Masao Yamasaki, Yusuke Omi, Naoko Fujii, Asako Ozaki, Akihiko Nakama, Yoichi Sakakibara, Masahito Suiko, Kazuo Nishiyama
• Journal: Bioscience, biotechnology, and biochemistry (Biosci Biotechnol Biochem) Vol. 73 Issue 10 Pg. 2217-21 (Oct 2009) ISSN: 1347-6947 [Electronic] England
• PMID: 19809176 (Publication Type: Journal Article)

Chemical References

• Plant Oils
• Reactive Oxygen Species
• ras Proteins
• Glutathione
• mustard oil

Topics

• Animals
• Cell Cycle (drug effects)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Cell Transformation, Neoplastic
• Glutathione (metabolism)
• Intracellular Space (genetics, metabolism)
• Mustard Plant
• Plant Oils (pharmacology)
• Raphanus (chemistry)
• Rats
• Reactive Oxygen Species (metabolism)
• ras Proteins (metabolism)