Effects of different general anesthetics on serum hemolysis and hepatic and muscular glycogenolysis in rats.

Abstract

Anesthetics can affect the structure and biological function of tissues and systems differentially. The aim of the present study was to compare three injectable anesthetics generally used in experiments with animals in terms of the degree of hemolysis and glycogenolysis occurring after profound anesthesia. Twenty-four male Wistar rats (330-440 g) were divided into three groups (N = 8): chloral hydrate (CH), ketamine + xylazine (KX), Zoletil 50(R) (zolazepam and tiletamine) + xylazine (ZTX). After deep anesthesia, total blood was collected. The liver and white (WG) and red gastrocnemius (RG) muscles were also immediately removed. The degree of serum hemolysis was quantified on the basis of hemoglobin concentration (g/L). Hepatic and muscular glycogen concentrations (mmol/kg wet tissue) were quantified by the phenol-sulfuric method. The CH and KX groups exhibited serum hemolysis (4.0 +/- 2.2 and 1.9 +/- 0.9 g/L, respectively; P < 0.05) compared to the ZTX group, which presented none. Only KX induced elevated glycogenolysis (mmol/kg wet tissue) in the liver (86.9 +/- 63.2) and in WG (18.7 +/- 9.0) and RG (15.2 +/- 7.2; P < 0.05). The CH and ZTX groups exhibited no glycogenolysis in the liver (164.4 +/- 41.1 and 176.8 +/- 54.4, respectively), WG (28.8 +/- 4.4, 32.0 +/- 6.5, respectively) or RG (29.0 +/- 4.9; 25.3 +/- 8.6, respectively). Our data indicate that ZTX seems to be an appropriate general anesthetic for studies that seek to simultaneously quantify the concentration of glycogen and serum biochemical markers without interferences. ZTX is reasonably priced, found easily at veterinary markets, quickly induces deep anesthesia, and presents a low mortality rate.

• Authors: E F A Machado, A C R Normand, L A S Nunes, R Brenzikofer, D V Macedo
• Journal: Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas (Braz J Med Biol Res) Vol. 42 Issue 11 Pg. 1035-8 (Nov 2009) ISSN: 1414-431X [Electronic] Brazil
• PMID: 19802466 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anesthetics, General
• Biomarkers
• Drug Combinations
• Liver Glycogen
• tiletamine, zolazepam drug combination
• Xylazine
• Tiletamine
• Ketamine
• Zolazepam

Topics

• Anesthetics, General (pharmacology)
• Animals
• Biomarkers (analysis)
• Drug Combinations
• Glycogenolysis (drug effects)
• Hemolysis (drug effects)
• Ketamine (pharmacology)
• Liver Glycogen (metabolism)
• Male
• Muscles (drug effects, enzymology)
• Rats
• Rats, Wistar
• Tiletamine (pharmacology)
• Xylazine (pharmacology)
• Zolazepam (pharmacology)