Association of clinicopathological features with UbcH10 expression in colorectal cancer.

Abstract

PURPOSE:

UbcH10 is the cancer-related E2 ubiquitin-conjugating enzyme, and its overexpression has been demonstrated in a variety of malignancies. The aim of this study is to investigate the association of UbcH10 gene expression with the carcinogenesis and tumor progression of colorectal cancer.

METHODS:

The expression levels of UbcH10 in human malignant colorectal carcinoma tissues and their adjacent normal tissues were examined using real-time quantitative RT-PCR and immunohistochemical analysis. The correlations of UbcH10 expression to the clinicalpathologic characteristics of the colorectal cancer were analyzed. Cell proliferation and Matrigel invasion assays were performed in HT-29 cells transfected with UbcH10 expression plasmid pcDNA3.1-UbcH10, UbcH10 RNA interference vector pUbcH10-RNAi as well as their control vectors.

RESULTS:

Our study demonstrated that the expression of UbcH10 in colorectal carcinoma tissues was significantly higher than that in non-cancerous tissues (P < 0.01), and the UbcH10 overexpression was related to the degree of tumor differentiation and lymph node metastasis of colorectal cancer patients (P < 0.05). In vitro, the overexpression of UbcH10 promoted cell proliferation and tumor invasiveness, but the downregulation of UbcH10 expression significantly reduced the growth rate and the invasiveness activity of tumor cell line.

CONCLUSIONS:

Our study suggests that the overexpression of UbcH10 gene plays a critical role in the carcinogenesis and tumor progression of colorectal cancer. It may be a new marker in diagnosis and prognosis of colorectal cancer, and the inhibition of UbcH10 may be a therapeutic potential for the treatment of colorectal cancer.

• Authors: Shimin Chen, Yingjian Chen, Chengjin Hu, Hongbiao Jing, Yongcheng Cao, Xianxi Liu
• Journal: Journal of cancer research and clinical oncology (J Cancer Res Clin Oncol) Vol. 136 Issue 3 Pg. 419-26 (Mar 2010) ISSN: 1432-1335 [Electronic] Germany
• PMID: 19779934 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• RNA, Small Interfering
• UBE2C protein, human
• Ubiquitin-Conjugating Enzymes

Topics

• Adult
• Aged
• Aged, 80 and over
• Carcinoma (diagnosis, genetics, pathology)
• Cell Differentiation (genetics)
• Cell Transformation, Neoplastic (genetics)
• Colorectal Neoplasms (diagnosis, genetics, pathology)
• Disease Progression
• Female
• Gene Expression Regulation, Neoplastic (drug effects)
• HT29 Cells
• Humans
• Male
• Middle Aged
• Prognosis
• RNA, Small Interfering (pharmacology)
• Tumor Burden (genetics)
• Ubiquitin-Conjugating Enzymes (antagonists & inhibitors, genetics, metabolism)
• Up-Regulation