Photoelectrochemical identification of 5-methylcytosine modification in DNA: combination of photosensitization and enzymatic cleavage.

Abstract

We present a novel photoelectrochemical approach for discriminating between cytosine and 5-methylcytosine in DNA when used in combination with enzymatic digestion. A photosensitizer-linked DNA duplex bearing 5-methylcytosine or cytosine at a given restriction site of the strand was immobilized on a gold electrode and digested with enzyme, and the photocurrent response was measured. We observed high photocurrent density that was selective for the methylated duplex.

• Authors: Kazuhito Tanabe, Hisatsugu Yamada, Takeo Ito, Sei-ichi Nishimoto
• Journal: Nucleic acids symposium series (2004) (Nucleic Acids Symp Ser (Oxf)) Issue 53 Pg. 205-6 ( 2009) ISSN: 1746-8272 [Electronic] England
• PMID: 19749332 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Oligodeoxyribonucleotides
• 5-Methylcytosine
• Cytosine
• DNA
• DNA Restriction Enzymes

Topics

• 5-Methylcytosine (analysis, chemistry)
• Cytosine (analysis)
• DNA (chemistry)
• DNA Methylation
• DNA Restriction Enzymes
• Oligodeoxyribonucleotides (chemistry)
• Photochemical Processes
• Photochemistry (methods)