Abstract | BACKGROUND: METHODS: Wistar albino rats, weighing 200-250 g, were allocated into three groups. After anesthetization with ketamine hydrochloride and xylazine, abdominal esophagus, gastroesophageal junction, and gastric fundus were removed via median laparotomy in the control group. In the oxygen (O(2)) group, a 16G catheter was inserted into the abdomen above the umbilicus and insufflated with 95% O(2) and 5% CO(2) with a pressure of 10 mm Hg. In the CO(2) group, CO(2) was insufflated at the same pressure within the same time and the tissues were removed at the end of a 60 min period of pneumoperitoneum. Abdominal esophageal segment (n:6), gastroesophageal junction (n:6) and gastric fundus (n:12) were suspended under 0.5 to 2 g resting tension in Tyrode solution in organ baths. Contraction responses were obtained by carbachol and serotonin and relaxation responses were evaluated by isoproterenol in each group. All the responses were compared by nonparametric Kruskal Wallis test. RESULTS:
Carbachol and serotonin induced contractile responses of abdominal segments, gastroesophageal junction, and gastric fundus showed no difference between the control, O(2), and CO(2) groups (P > 0.05). Isoproterenol relaxation responses of the three groups were also not statistically different from each other (P > 0.05). CONCLUSION: CO(2) pneumoperitoneum of 60 min has no influence on esophageal and gastric smooth muscle responses to different agonists in rats.
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Authors | Tutku Soyer, Zuhal Aktuna, Tolga Reşat Aydos, Gökhan Osmanoğlu, Oğuzhan Korkut, Hülya Akman, Murat Cakmak |
Journal | The Journal of surgical research
(J Surg Res)
Vol. 161
Issue 2
Pg. 278-81
(Jun 15 2010)
ISSN: 1095-8673 [Electronic] United States |
PMID | 19524263
(Publication Type: Journal Article)
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Copyright | Copyright 2010 Elsevier Inc. All rights reserved. |
Chemical References |
- Carbon Dioxide
- Serotonin
- Carbachol
- Isoproterenol
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Topics |
- Abdomen
(physiopathology)
- Animals
- Carbachol
(pharmacology)
- Carbon Dioxide
(pharmacology)
- Esophagus
(drug effects, physiopathology)
- Isoproterenol
(pharmacology)
- Laparotomy
(methods)
- Muscle Contraction
(drug effects)
- Muscle Relaxation
(drug effects)
- Muscle, Smooth
(drug effects, physiopathology)
- Pneumoperitoneum
(chemically induced, physiopathology)
- Rats
- Rats, Wistar
- Serotonin
(pharmacology)
- Stomach
(drug effects, physiopathology)
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