Identification of the Arabidopsis dry2/sqe1-5 mutant reveals a central role for sterols in drought tolerance and regulation of reactive oxygen species.
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| Abstract |
Squalene epoxidase enzymes catalyse the conversion of squalene into 2,3-oxidosqualene, the precursor of cyclic triterpenoids. Here we report that the Arabidopsis drought hypersensitive/squalene epoxidase 1-5 (dry2/sqe1-5) mutant, identified by its extreme hypersensitivity to drought stress, has altered stomatal responses and root defects because of a point mutation in the SQUALENE EPOXIDASE 1 (SQE1) gene. GC-MS analysis indicated that the dry2/sqe1-5 mutant has altered sterol composition in roots but wild-type sterol composition in shoots, indicating an essential role for SQE1 in root sterol biosynthesis. Importantly, the stomatal and root defects of the dry2/sqe1-5 mutant are associated with altered production of reactive oxygen species. As RHD2 NADPH oxidase is de-localized in dry2/sqe1-5 root hairs, we propose that sterols play an essential role in the localization of NADPH oxidases required for regulation of reactive oxygen species, stomatal responses and drought tolerance.
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| Authors | David Posé, Itziar Castanedo, Omar Borsani, Benjamín Nieto, Abel Rosado, Ludivine Taconnat, Albert Ferrer, Liam Dolan, Victoriano Valpuesta, Miguel A Botella |
| Journal | The Plant journal : for cell and molecular biology (Plant J) Vol. 59 Issue 1 Pg. 63-76 (Jul 2009) ISSN: 1365-313X [Electronic] England |
| PMID | 19309460 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
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