Abstract |
Studies have shown that exogenous nerve growth factor ( NGF) accelerates ulcer healing, but the inefficient growth factor delivery system limits its clinical application. In this report, we found that the native human NGF-beta fused with a collagen-binding domain (CBD) could form a collagen-based NGF targeting delivery system, and the CBD-fused NGF-beta could bind to collagen membranes efficiently. Using the rabbit dermal ischemic ulcer model, we have found that this targeting delivery system maintains a higher concentration and stronger bioactivity of NGF-beta on the collagen membranes by promoting peripheral nerve growth. Furthermore, it enhances the rate of ulcer healing through accelerating the re-epithelialization of dermal ulcer wounds and the formation of capillary lumens within the newly formed tissue area. Thus, collagen membranes loaded with collagen-targeting human NGF-beta accelerate ulcer healing efficiently.
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Authors | Wenjie Sun, Hang Lin, Bing Chen, Wenxue Zhao, Yannan Zhao, Zhifeng Xiao, Jianwu Dai |
Journal | Journal of biomedical materials research. Part A
(J Biomed Mater Res A)
Vol. 92
Issue 3
Pg. 887-95
(Mar 01 2010)
ISSN: 1552-4965 [Electronic] United States |
PMID | 19283824
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | (c) 2009 Wiley Periodicals, Inc. |
Chemical References |
- Collagen
- Nerve Growth Factor
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Topics |
- Animals
- Collagen
(metabolism)
- Enzyme-Linked Immunosorbent Assay
- Immunohistochemistry
- Microscopy, Electron, Scanning
- Nerve Growth Factor
(metabolism)
- Protein Binding
- Rabbits
- Skin Ulcer
(metabolism)
- Wound Healing
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