Erythropoietin inhibits the increase of intestinal labile zinc and the expression of inflammatory mediators after traumatic brain injury in rats.

Abstract

BACKGROUND:

The objective of this study was to determine the effect of erythropoietin (Epo) on the intestinal labile zinc and the inflammatory factor in rats after traumatic brain injury (TBI).

METHODS:

Male Sprague-Dawley rats were randomly divided into nine groups: (a) normal group; (b) sham-operation group; (c, d, e, f, and g) TBI group, killed at 1 hour, 6 hour, 24 hour, and 72 hour and 7 days postinjury, respectively; (h and i) TBI + saline and TBI + Epo, killed at 24 hour or 72 hour postinjury. Parietal brain contusion was produced by a free-falling weight on the exposed dura of the right parietal lobe. Intestinal labile zinc, the tumor necrosis factor-alpha, interleukin (IL)-8, and wet/dry weight ratio were investigated in different groups.

RESULTS:

The gut contains a certain amount of labile zinc in normal animals and TBI caused obviously gradual increment of intestinal liabled zinc. The levels of inflammatory mediators and the gut wet/dry weight ratio were also found to increase in the trauma group (p < 0.05). There was a highly positive correlation between the abundance of zinc fluorescence and these proinflammation factors. Epo significantly reduced the intestinal labile zinc, the inflammatory mediators, and the gut wet/dry weight ratio compared with TBI group (p < 0.05).

CONCLUSIONS:

Epo can protect intestine from TBI-induced injury by attenuating intestinal inflammation and labile zinc accumulation in vivo.

• Authors: Lin Zhu, Wei Jin, Hao Pan, Zelan Hu, Jing Zhou, Chunhua Hang, Jixin Shi
• Journal: The Journal of trauma (J Trauma) Vol. 66 Issue 3 Pg. 730-6 (Mar 2009) ISSN: 1529-8809 [Electronic] United States
• PMID: 19276746 (Publication Type: Journal Article)

Chemical References

• Inflammation Mediators
• Interleukin-8
• Recombinant Proteins
• Tumor Necrosis Factor-alpha
• Erythropoietin
• Zinc

Topics

• Animals
• Brain Concussion (pathology, physiopathology)
• Capillary Permeability (drug effects, physiology)
• Enzyme-Linked Immunosorbent Assay
• Erythropoietin (pharmacology)
• Homeostasis (drug effects, physiology)
• Inflammation Mediators (blood)
• Interleukin-8 (blood)
• Intestinal Mucosa (drug effects, pathology, physiopathology)
• Male
• Microscopy, Fluorescence
• Parietal Lobe (injuries)
• Rats
• Rats, Sprague-Dawley
• Recombinant Proteins
• Systemic Inflammatory Response Syndrome (pathology, physiopathology)
• Tumor Necrosis Factor-alpha (metabolism)
• Zinc (blood)