Abstract | OBJECTIVE: METHODS: The tissues of keloid, hypertrophic scar and normal skin were tested. RT-PCR was used to assess the mRNA expression levels of the aimed genes. The distribution of fibrillin 1 in scars and normal skin was examined by immunohistochemistry staining. RESULTS: The mRNA level of fibrillin 1 in keloid (0.802 +/- 0.116) was increased by 218.25% (P < 0.01) than that in normal skin (0.252 +/- 0.067). The expression of the gene in hypertrophic scar (0.628 +/- 0.144) was higher by 149.21% (while, P > 0.05) than that in normal skin. The expression of TGF-beta1 in keloid and hypertrophic scar were more than that in normal skin. The expression of fibrillin 1 was related to that of TGF-beta1 positively (r = 0.820, P < 0.01). Fibrillin 1 protein was stained positively in basic membranes, endothelial cells, fibroblasts and extracellular matrix of skin tissues. In dermal, the protein levels of fibrillin 1 in keloid (0.117 +/- 0.042) was decreased than those in normal skin (0.185 +/- 0.043) and hypertrophic scar (0.181 +/- 0.048), the inhibition rates were 36.76%, 35.36% respectively (both P < 0.01). CONCLUSIONS:
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Authors | Fang-Fei Nie, Qi Wang, Ze-Lian Qin |
Journal | Zhonghua zheng xing wai ke za zhi = Zhonghua zhengxing waike zazhi = Chinese journal of plastic surgery
(Zhonghua Zheng Xing Wai Ke Za Zhi)
Vol. 24
Issue 5
Pg. 339-42
(Sep 2008)
ISSN: 1009-4598 [Print] China |
PMID | 19119630
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- FBN1 protein, human
- Fibrillin-1
- Fibrillins
- Microfilament Proteins
- RNA, Messenger
- Transforming Growth Factor beta1
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Topics |
- Cicatrix, Hypertrophic
(metabolism, pathology)
- Fibrillin-1
- Fibrillins
- Humans
- Keloid
(metabolism, pathology)
- Microfilament Proteins
(metabolism)
- RNA, Messenger
(genetics)
- Transforming Growth Factor beta1
(genetics, metabolism)
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