A simple and fast liquid-liquid extraction method for the determination of 2,2',4,4',5,5'-hexachlorobiphenyl (CB-153) and 1,1-dichloro-2,2-bis(p-chlorophenyl)-ethylene (p,p'-DDE) from human serum for epidemiological studies on type 2 diabetes.

Abstract

A simple and fast method is presented to be used for example in studies on the relationship between serum levels of persistent organic pollutants and type 2 diabetes mellitus. Method is based on liquid-liquid extraction and gas chromatography coupled with high-resolution mass spectrometry. In the sample pre-treatment special attention was paid to minimize the number of sample manipulation steps and the amounts of organic solvents needed. Compounds analyzed were 2,2',4,4',5,5'-hexachlorobiphenyl (PCB-153) and 1,1-dichloro-2,2-bis (p-chlorophenyl)-ethylene (p,p'-DDE), the major metabolite of DDT. The method included extraction and cleanup of 0.2ml of serum in a single test tube and subsequent analysis of the extract from 0.2ml final volume. Validation was conducted to explore the performance of the method. The limits of detection for p,p'-DDE and PCB-153 based on the standard deviation of the blank samples were 4.3 and 3.1pg/ml, respectively. Repeatability was less than 2.5% at three concentration levels tested and recovery from Certified Reference Material SRM 1589a was 84% for p,p'-DDE and 87% for PCB-153 of the certified values, respectively. Serum samples from the AMAP intercalibration round 2008-2 were also analyzed, and results were 101-116% of the assigned values. The presented method was used for an epidemiological study with more than 700 serum samples from a type 2 diabetes cohort from Sweden.

• Authors: Panu Rantakokko, Hannu Kiviranta, Lars Rylander, Anna Rignell-Hydbom, Terttu Vartiainen
• Journal: Journal of chromatography. A (J Chromatogr A) Vol. 1216 Issue 6 Pg. 897-901 (Feb 06 2009) ISSN: 0021-9673 [Print] Netherlands
• PMID: 19108840 (Publication Type: Journal Article)

Chemical References

• Environmental Pollutants
• Dichlorodiphenyl Dichloroethylene
• Polychlorinated Biphenyls
• 2,4,5,2',4',5'-hexachlorobiphenyl

Topics

• Analysis of Variance
• Chemical Fractionation (methods)
• Diabetes Mellitus, Type 2 (blood, epidemiology)
• Dichlorodiphenyl Dichloroethylene (analogs & derivatives, analysis, blood)
• Environmental Pollutants (blood)
• Gas Chromatography-Mass Spectrometry
• Humans
• Polychlorinated Biphenyls (analysis, blood)
• Reproducibility of Results
• Sensitivity and Specificity