Inhibitory effect of matrine on the expression of PSA and AR in prostate cancer cell line LNCaP.

Abstract

In order to investigate the inhibitory effect of matrine on the expression of prostate specific antigen (PSA) and androgen receptor (AR) in prostate cancer cell line LNCaP in vitro, LNCaP cells were treated with matrine at different concentrations (0.5, 1.0, 1.5, 2.0 g/L) for 12-36 h. The growth activities of cancer cells were determined by MTT colorimetric assay. The AR level was measured by Western blotting. The expression of PSA was detected by using AXSYM system-chemical luciferase methods. The results showed that matrine could effectively inhibit the growth of androgen-dependent prostate cancer cell line LNCaP in vitro in a time- and dose-dependent manner (P<0.05). It could obviously decrease the level of AR (P<0.01) and inhibit the expression of PSA in a dose-dependent manner (P<0.05) in LNCaP cells. It was concluded that matrine could significantly suppress the growth of LNCaP cells and inhibit the expression of PSA and AR of prostate cancer cells.

• Authors: Ke Chen, Zhiquan Hu, Tao Wang, Hui Guo, Zhangqun Ye
• Journal: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban (J Huazhong Univ Sci Technolog Med Sci) Vol. 28 Issue 6 Pg. 697-9 (Dec 2008) ISSN: 1672-0733 [Print] China
• PMID: 19107370 (Publication Type: Journal Article)

Chemical References

• Alkaloids
• Antineoplastic Agents, Phytogenic
• Quinolizines
• Receptors, Androgen
• Prostate-Specific Antigen
• Matrines

Topics

• Alkaloids (pharmacology)
• Antineoplastic Agents, Phytogenic (pharmacology)
• Cell Line, Tumor
• Dose-Response Relationship, Drug
• Humans
• Male
• Prostate-Specific Antigen (genetics, metabolism)
• Prostatic Neoplasms (metabolism, pathology)
• Quinolizines (pharmacology)
• Receptors, Androgen (genetics, metabolism)
• Matrines