[Recombinant envelope glycoprotein domain III of dengue virus inhibit virus infection].

Abstract

OBJECTIVE:

To observe the ability of dengue virus recombinant envelope protein domain expressed in E. coli to inhibit virus infection and induce the neutralizing antibody.

METHODS:

E III protein of Dengue virus serotypes 1-4 were expressed in E. coli BL21(DE3) then purified. Recombinant proteins were tested to inhibit DV2 from infecting BHK-21 cell. Rabbits were immunized with recombinant proteins to produce anti-E III serum. Antibody titers were determined by neutralizing assay.

RESULTS:

The recombinant E III proteins of Dengue virus serotypes 1-4 were expressed in E. coli. They effectively protected BHK cells in culture against DV2 infection. All four type anti-E III sera can neutralize DV2 but their efficacies are different.

CONCLUSION:

proteins of dengue virus expressed in E. coli can directly inhibit DV2 infection. Neutralizing antibodies were induced by E III proteins. Both E III protein of dengue virus and the neutralizing antibodies they induced are more efficient in inhibiting homologous dengue serotypes infection than heterologous serotypes.

• Authors: Peng Lu, Yan Wei, Shou-Chun Cao, Jian-Dong Li, Qin-Zhi Liu, Quan-Fu Zhang, Chuan Li, Fang Miao, Shuo Zhang, Xiao-Tong Hang, Mi-Fang Liang, De-Xin Li
• Journal: Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology (Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi) Vol. 22 Issue 3 Pg. 177-9 (Jun 2008) ISSN: 1003-9279 [Print] China
• PMID: 19031695 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Viral
• Recombinant Proteins
• Viral Envelope Proteins

Topics

• Animals
• Antibodies, Viral (immunology)
• Cell Line
• Cricetinae
• Dengue (immunology, prevention & control, virology)
• Dengue Virus (chemistry, genetics, immunology, physiology)
• Down-Regulation
• Escherichia coli (genetics, metabolism)
• Humans
• Immunization
• Mesocricetus
• Protein Structure, Tertiary
• Rabbits
• Recombinant Proteins (chemistry, genetics, immunology)
• Viral Envelope Proteins (chemistry, genetics, immunology)
• Virus Replication