[Effects of nuclear factor-kappa B p65 ASODN on transforming growth beta-1 and intercellular adhesion molecule-1 of rat hepatic stellate cells].

Abstract

OBJECTIVE:

To study the effects of nuclear factor (NF)-kappa B p65 ASODN on transforming growth factor beta-1 (TGF beta 1) and intercellular adhesion molecule-1 (ICAM-1) of rat hepatic stellate cells (HSC) and the mechanisms of NF-kappa B p65 ASODN in treating liver fibrosis.

METHODS:

Type IV collagen enzyme digestion and density centrifugation methods were used to separate rat hepatic stellate cells. NF-kappa B p65 ASODN was manually synthesized and completely phosphorothioate-modified. The changes of TGF beta 1 and ICAM-1 mRNA were detected by RT-PCR and albumen of TGF beta 1 and ICAM-1 were detected by ELISA. The changes of NF-kappa B activity were determined by ELISA.

RESULTS:

NF-kappa B activity and the expressions of ICAM-1 and TGF beta 1 increased after the HSC were treated by TNF alpha. NF-kappa B activity weakened after being treated with NF-kappa B p65 ASODN (0.001-1.000 micromol/L), P less than 0.05 in a dose dependent manner. Transferring NF-kappa B p65 ASODN (0.001-1.000 micromol/L) also weakened the expression of ICAM-1 and TGF beta 1 mRNA and the protein induced by TNF alpha in HSC. It was also in a dose dependent manner, P less than 0.05.

CONCLUSIONS:

After transferring NF-kappa B p65 ASODN into HSC, their NF-kappa B activity decreased, and their mRNA and protein expressions of ICAM-1 and TGF beta 1 also decreased. This may serve as a new way in treating hepatic fibrosis.

• Authors: Yu Zhou, Zhi-ling Tang, Guo-li Wei, Hao Wang, Shi-cai Ye, Ke-quan Chen, Yuan-hong Chen, Rong-huo Liu
• Journal: Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology (Zhonghua Gan Zang Bing Za Zhi) Vol. 16 Issue 10 Pg. 762-6 (Oct 2008) ISSN: 1007-3418 [Print] China
• PMID: 18983774 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Oligonucleotides, Antisense
• Rela protein, rat
• Transcription Factor RelA
• Transforming Growth Factor beta1
• Tumor Necrosis Factor-alpha
• Intercellular Adhesion Molecule-1

Topics

• Animals
• Cell Line
• Hepatic Stellate Cells (metabolism)
• Intercellular Adhesion Molecule-1 (metabolism)
• Male
• Oligonucleotides, Antisense
• Rats
• Rats, Sprague-Dawley
• Transcription Factor RelA (genetics)
• Transforming Growth Factor beta1 (metabolism)
• Tumor Necrosis Factor-alpha (pharmacology)