[Effects of proteasome inhibitor bortezomib on NF-kappaB activity and ICAM-1 mRNA expression of K562 cells].

Abstract

This study was aimed to investigate the effects of proteasome inhibitor bortezomib (Velcade, PS-341) on the activation of NF-kappaB and the expression of intercellular adhesion molecule-1 (ICAM-1) in K562 cells. The K562 cells were incubated in the culture of RPMI 1640 with 10% calf serum in 12-well plates and exposed to 0, 10, 20, 30, 50 and 100 nmol/L of bortezomib for 6 hours. The activation of NF-kappaB was analyzed by SP immunohistochemistry, meanwhile RT-PCR was performed to detect expression of ICAM-1. The results showed that the activation of NF-kappaB and the expression of ICAM-1 in K562 cells decreased significantly after bortezomib treatment. The inhibitory effect on ICAM-1 was probably related with the activity suppression of NF-kappaB. It is concluded that proteasome inhibitor bortezomib downregulates the expression of K562 cell ICAM-1 by inhibiting the activity of NF-kappaB, which provides a new way for the target therapy in acute leukemia.

• Authors: Shi-Feng Lu, Hua Lu, Wen-Yi Shen, Jian-Fu Zhang, Peng Liu, Yong-Ren Wang, Li-Xia Wang, Hui Yang, Jian-Yong Li
• Journal: Zhongguo shi yan xue ye xue za zhi (Zhongguo Shi Yan Xue Ye Xue Za Zhi) Vol. 16 Issue 5 Pg. 1006-9 (Oct 2008) ISSN: 1009-2137 [Print] China
• PMID: 18928584 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Boronic Acids
• NF-kappa B
• Protease Inhibitors
• Pyrazines
• Intercellular Adhesion Molecule-1
• Bortezomib

Topics

• Boronic Acids (pharmacology)
• Bortezomib
• Humans
• Intercellular Adhesion Molecule-1 (metabolism)
• K562 Cells
• NF-kappa B (metabolism)
• Protease Inhibitors (pharmacology)
• Pyrazines (pharmacology)