[Chaiqin Chengqi Decoction decreases pancreatic acinar cell calcium overload in rats with acute pancreatitis].

Abstract

OBJECTIVE:

To investigate the mechanism of Chaiqin Chengqi Decoction (CQCQD), a compound of traditional Chinese herbal medicine, acting on the pancreatic acinar cell calcium overload in rats with acute pancreatitis (AP).

METHODS:

A total of 30 SD rats were randomly divided into normal control group, untreated group and CQCQD group (n=10, respectively). AP was induced in rats by caerulein (5x50 mug/kg) intraperitoneal injection within 4 h. The pancreatic tissue SERCA1 and SERCA2 mRNA expressions were detected by fluorescent quantization polymerase chain reaction method; intracellular calcium fluorescence intensity (FI) of pancreatic acinar cells and the pancreatic pathological score were measured by laser scanning confocal microscopy and light microscopy respectively.

RESULTS:

There were no SERCA1 mRNA expressions in pancreatic acinar cells of rats in the normal control group and the untreated group. The expression of pancreatic SERCA2 mRNA in the untreated group was down-regulated compared with that in the normal control group (expression ratio=0.536; P=0.001); the expression of pancreatic SERCA2 mRNA in the CQCQD group was up-regulated compared with that in the untreated group (expression ratio=2.00; P=0.012). The pancreatic pathological score in the CQCQD group was lower than that in the untreated group and the FI of Ca(2+) was also lower.

CONCLUSION:

CQCQD can up-regulate the expression of pancreatic SERCA2 mRNA, release the calcium overload, and hence reduce the pathological changes in pancreatic tissue.

• Authors: Ping Xue, Li-Hui Deng, Zhao-da Zhang, Xiao-Nan Yang, Qing Xia, Da-Kai Xiang, Lei Huang, Mei-Hua Wan, Hai-Yan Zhang
• Journal: Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine (Zhong Xi Yi Jie He Xue Bao) Vol. 6 Issue 10 Pg. 1054-8 (Oct 2008) ISSN: 1672-1977 [Print] China
• PMID: 18847542 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Atp2a2 protein, rat
• Calcium Channel Blockers
• Chai Qin Cheng Qi decoction
• Drugs, Chinese Herbal
• RNA, Messenger
• Ceruletide
• Sarcoplasmic Reticulum Calcium-Transporting ATPases
• Calcium

Topics

• Acute Disease
• Animals
• Calcium (metabolism)
• Calcium Channel Blockers (pharmacology, therapeutic use)
• Ceruletide
• Drugs, Chinese Herbal (pharmacology, therapeutic use)
• Male
• Pancreas, Exocrine (metabolism, pathology)
• Pancreatitis (chemically induced, drug therapy, metabolism)
• RNA, Messenger (genetics, metabolism)
• Random Allocation
• Rats
• Rats, Sprague-Dawley
• Sarcoplasmic Reticulum Calcium-Transporting ATPases (genetics, metabolism)