Differential membrane proteomics using 18O-labeling to identify biomarkers for cholangiocarcinoma.

Abstract

Quantitative proteomic methodologies allow profiling of hundreds to thousands of proteins in a high-throughput fashion. This approach is increasingly applied to cancer biomarker discovery to identify proteins that are differentially regulated in cancers. Fractionation of protein samples based on enrichment of cellular subproteomes prior to mass spectrometric analysis can provide increased coverage of certain classes of molecules. We used a membrane protein enrichment strategy coupled with 18O labeling based quantitative proteomics to identify proteins that are highly expressed in cholangiocarcinomas. In addition to identifying several proteins previously known to be overexpressed in cholangiocarcinoma, we discovered a number of molecules that were previously not associated with cholangiocarcinoma. Using immunoblotting and immunohistochemical labeling of tissue microarrays, we validated Golgi membrane protein 1, Annexin IV and Epidermal growth factor receptor pathway substrate 8 (EPS8) as candidate biomarkers for cholangiocarcinomas. Golgi membrane protein 1 was observed to be overexpressed in 89% of cholangiocarcinoma cases analyzed by staining tissue microarrays. In light of recent reports showing that Golgi membrane protein 1 is detectable in serum, further investigation into validation of this protein has the potential to provide a biomarker for early detection of cholangiocarcinomas.

• Authors: Troels Zakarias Kristiansen, H C Harsha, Mads Grønborg, Anirban Maitra, Akhilesh Pandey
• Journal: Journal of proteome research (J Proteome Res) Vol. 7 Issue 11 Pg. 4670-7 (Nov 2008) ISSN: 1535-3893 [Print] United States
• PMID: 18839982 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor
• Oxygen Isotopes
• Proteome

Topics

• Bile Duct Neoplasms (genetics, pathology)
• Bile Ducts, Intrahepatic (pathology)
• Biomarkers, Tumor (analysis, genetics)
• Case-Control Studies
• Cellular Structures (chemistry)
• Cholangiocarcinoma (genetics, pathology)
• Humans
• Immunohistochemistry
• Isotope Labeling
• Membranes (chemistry)
• Microarray Analysis
• Oxygen Isotopes
• Proteome (analysis)
• Proteomics (methods)