Collagenolytic fragments and active gelatinase complexes in periodontitis.

Abstract

BACKGROUND:

Periodontal tissues remodel rapidly, which enables quick adaptation to mechanical changes. Matrix metalloproteinases (MMPs) are involved in these remodeling processes under control of tissue inhibitor of metalloproteinases (TIMPs). In periodontitis, overactivity of MMPs results in pathologic tissue degradation. The aim of this study was to analyze MMPs and TIMPs in healthy and diseased gingiva, periodontal ligament (PDL), and gingival crevicular fluid (GCF).

METHODS:

Samples of gingiva, PDL, and GCF were obtained from healthy controls (gingiva: n = 18; PDL: n = 15; GCF: n = 8) and subjects with periodontitis (gingiva: n = 11; PDL: n = 18; GCF: n = 12). MMPs and TIMPs were analyzed by gelatin-, collagen-, and reverse zymography and by Western blotting. Total MMP activity was analyzed using a fluorogenic substrate.

RESULTS:

TIMP-1 and -2, active and pro-MMP-2 and -9, and active MMP-1 and -8 were present in all samples. Large amounts of active MMP-2 complexes and collagenolytic fragments were also found. Their levels were higher in PDL and GCF from subjects with periodontitis. In general, TIMP levels were lower in diseased periodontal tissues. Especially diseased GCF contained more MMPs. Surprisingly, some MMPs were more abundant in healthy gingiva and PDL than in diseased tissue.

CONCLUSIONS:

Unexpected variations in MMP and TIMP levels in gingiva, PDL, and GCF may result from differences in subject characteristics and disease activity. The levels of active MMP-2 complexes and collagenolytic fragments are higher in the periodontium of subjects with periodontitis and might contribute significantly to periodontal destruction.

• Authors: Miriam M Bildt, Marjon Bloemen, Anne M Kuijpers-Jagtman, Johannes W Von den Hoff
• Journal: Journal of periodontology (J Periodontol) Vol. 79 Issue 9 Pg. 1704-11 (Sep 2008) ISSN: 0022-3492 [Print] United States
• PMID: 18771372 (Publication Type: Journal Article)

Chemical References

• Fluorescent Dyes
• Tissue Inhibitor of Metalloproteinase-1
• Tissue Inhibitor of Metalloproteinases
• Tissue Inhibitor of Metalloproteinase-2
• Matrix Metalloproteinases
• MMP2 protein, human
• Matrix Metalloproteinase 2
• MMP8 protein, human
• Matrix Metalloproteinase 8
• Matrix Metalloproteinase 9
• MMP1 protein, human
• Matrix Metalloproteinase 1

Topics

• Adolescent
• Adult
• Aged
• Blotting, Western
• Female
• Fluorescent Dyes
• Gingiva (enzymology)
• Gingival Crevicular Fluid (enzymology)
• Humans
• Male
• Matrix Metalloproteinase 1 (analysis)
• Matrix Metalloproteinase 2 (analysis)
• Matrix Metalloproteinase 8 (analysis)
• Matrix Metalloproteinase 9 (analysis)
• Matrix Metalloproteinases (analysis)
• Middle Aged
• Periodontal Ligament (enzymology)
• Periodontitis (enzymology)
• Tissue Inhibitor of Metalloproteinase-1 (analysis)
• Tissue Inhibitor of Metalloproteinase-2 (analysis)
• Tissue Inhibitor of Metalloproteinases (analysis)