Potential biomarkers of acute cerebral infarction detected by SELDI-TOF-MS.

Abstract

No single biologic marker is used in the routine diagnosis of acute cerebral infarction. We screened for potential biomarkers in 92 plasma samples, including samples from 32 patients with acute cerebral infarction and 60 hospital control subjects. Pretreated plasma samples were analyzed using SELDI-TOF-MS (Ciphergen Biosystems, Fremont, CA). Proteomic spectra of mass to charge ratio (m/z) were generated by the application of plasma to weak cation exchange (CM10 ProteinChip, Ciphergen Biosystems) arrays. A differential pattern consisting of 13 biomarkers was selected based on their collective contribution to the optimal separation between patients with acute cerebral infarction and control subjects with a sensitivity of 84.4% and specificity of 95.0%, respectively. Plasma proteomic profiling with SELDI-TOF-MS and ProteinChip technologies shows potential in discriminating patients with acute cerebral infarction and control subjects. Diagnosis of acute cerebral infarction should probably depend on the use of a panel of biomarkers.

• Authors: Xiaoping Zhang, Tao Guo, Huafang Wang, Wenjuan He, Heng Mei, Mei Hong, Jiekai Yu, Yu Hu, Shanjun Song
• Journal: American journal of clinical pathology (Am J Clin Pathol) Vol. 130 Issue 2 Pg. 299-304 (Aug 2008) ISSN: 0002-9173 [Print] England
• PMID: 18628101 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers

Topics

• Acute Disease
• Aged
• Biomarkers (analysis)
• Cerebral Infarction (diagnosis)
• Computational Biology
• Female
• Humans
• Male
• Middle Aged
• Protein Array Analysis
• Proteomics
• Reproducibility of Results
• Sensitivity and Specificity
• Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization