[Bystander effect of target-regulated uracil phosphoribosyltransferase/5-fluorouracil suicide gene system on prostate cancer cell].

Abstract

OBJECTIVE:

To study the bystander effect of Uracil Phosphoribosyltransferase (UPRT )/5-fluorouracil (5-FU) suicide gene system, which is regulated by PSMA enhancer/promoter, on prostate cancer cell.

METHODS:

Mediated by liposome, pPSMA enhancer/promoter-UPRT was transferred into prostate cancer line. And the cell line that could express UPRT gene stably was selected with antibiotic G418. The various percentages of prostate cancer cells (LNCaP) transfected and non-transfected were mixed to observe the bystander effect of suicide gene system UPRT/5-FU.

RESULTS:

The cell line that could express UPRT gene stably was screened successfully. With the LNCaP cells that expressed UPRT gene becoming more and more, the cell survival rate went down gradually. No obvious bystander effect showed after using UPRT/5-FU suicide gene system.

CONCLUSION:

pPSMA enhancer/promoter-UPRT/5-FU suicide gene system kills LNCaP cells in target, UPRT can transform 5-FU into toxic metabolites quickly and play the role of cytotoxicity. However, the bystander effect of UPRT/5-FU isn't obvious. It will be studied further for UPRT/5-FU how to strengthen the cytotoxic role by improving the bystander effect.

• Authors: Fu-Jun Zhao, Hong Li, Hao Zeng, Qiang Wei, Xiang Li
• Journal: Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition (Sichuan Da Xue Xue Bao Yi Xue Ban) Vol. 39 Issue 3 Pg. 394-7 (May 2008) ISSN: 1672-173X [Print] China
• PMID: 18575323 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antimetabolites, Antineoplastic
• Pentosyltransferases
• uracil phosphoribosyltransferase
• Fluorouracil

Topics

• Antimetabolites, Antineoplastic (pharmacology)
• Bystander Effect
• Cell Line, Tumor
• Cell Survival (drug effects, genetics, physiology)
• Fluorouracil (pharmacology)
• Genes, Transgenic, Suicide (genetics)
• Humans
• Male
• Pentosyltransferases (genetics, metabolism)
• Prostatic Neoplasms (genetics, metabolism, pathology)