Abstract |
The present study examined the hypothesis that cerebral ischemic tolerance induced by hyperbaric oxygen preconditioning (HBO-PC) is associated with an increase of antioxidant enzyme activity. Male Sprague-Dawley rats (250-280 g, n=74) were divided into sham, middle cerebral artery occlusion (MCAO) for 90 min, and MCAO plus HBO-PC groups. HBO-PC was conducted four times by given 100% oxygen at 2.5 atmosphere absolute (ATA), for 1 h at every 12 h interval for 2 days. At 24 h after the last HBO-PC, MCAO was performed and at 24 h after MCAO, neurological function and Nissl Staining were performed to evaluate the effect of HBO-PC. Malondialdehyde (MDA) content, activity of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-px) sampled from the hippocampus, ischemic penumbra or core of cortex were measured. HBO-PC decreased mortality rate, improved neurological recovery, lessened neuronal injury, reduced the level of MDA and increased the antioxidant activity of CAT and SOD. These observations demonstrated that an upregulation of the antioxidant enzyme activity by HBO preconditioning plays an important role in the generation of tolerance against brain ischemia- reperfusion injury.
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Authors | Jiasi Li, Wenwu Liu, Suju Ding, Weigang Xu, Yangtai Guan, John H Zhang, Xuejun Sun |
Journal | Brain research
(Brain Res)
Vol. 1210
Pg. 223-9
(May 19 2008)
ISSN: 0006-8993 [Print] Netherlands |
PMID | 18407255
(Publication Type: Journal Article)
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Chemical References |
- Antioxidants
- Enzymes
- Reactive Oxygen Species
- Malondialdehyde
- Catalase
- Sod1 protein, rat
- Superoxide Dismutase
- Superoxide Dismutase-1
- Glutathione
- Oxygen
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Topics |
- Animals
- Antioxidants
(metabolism)
- Brain
(enzymology)
- Brain Ischemia
(enzymology, therapy)
- Catalase
(metabolism)
- Enzyme Activation
- Enzymes
(metabolism)
- Glutathione
(metabolism)
- Hyperbaric Oxygenation
- Infarction, Middle Cerebral Artery
(enzymology, therapy)
- Ischemic Preconditioning
- Male
- Malondialdehyde
(metabolism)
- Oxidative Stress
- Oxygen
(metabolism, pharmacology, therapeutic use)
- Oxygen Consumption
(physiology)
- Rats
- Rats, Sprague-Dawley
- Reactive Oxygen Species
(metabolism)
- Reperfusion Injury
(enzymology, therapy)
- Superoxide Dismutase
(metabolism)
- Superoxide Dismutase-1
- Up-Regulation
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